中文摘要：

目的探讨踏转轮运动训练对新生期大鼠反复惊厥所致学习能力损害及海马ZnT3表达的影响。方法将出生后6d的SD大鼠随机分为惊厥组和对照组。惊厥组每日吸入三氟乙醚诱导惊厥发作1次，每次持续30min，连续6d；对照组给予同样操作，但期间不吸入三氟乙醚。2组大鼠分别于出生后29～35d及61—67d进行Y迷宫学习训练，检测其学习、记忆功能。期间2组大鼠于出生后51～56d进行踏转轮训练，每天1次，每次30min，连续6d。最后于出生后78d时将各组大鼠处死行脑组织切片，检测ZnT3在海马中的表达。结果①学习能力测试：惊厥组大鼠第1次Y-迷宫辨别学习达标的电刺激次数为（60．0±14．1）次，与对照组[（37．5±17．2）次]比较，差异有统计学意义（P〈0．05）；第2次惊厥组Y-迷宫辨别学习达标的电刺激次数为（27．5±14．1）次，与对照组[（21．0±11．0）次]比较，差异无统计学意义（P〉0．05）；2组大鼠第2次学习能力成绩均较第1次显著改善，差异均有统计学意义（P〈0．05）。②记忆能力测试：惊厥组与对照组2次测试结果间差异均无统计学意义（P〉0．05）。③ZnT3原位杂交检测：2组大鼠ZnT3 mRNA在海马各区均有明显表达，差异无统计学意义（P〉0．05）；但惊厥组大鼠齿状回znT3 mRNA灰度值与CA3区比值较对照组显著减小，差异具有统计学意义（P〈0．05）。结论运动训练能显著改善新生期反复长程惊厥对大鼠学习能力的榻喜．并能有效调节海马氏ZnT3的异常表扶水平．

英文摘要：

Objective To explore the long-term effects of physical exercise on neonatal seizure-induced learning, memory deficit and the expression of zinc transporter-3 （ZnT3） in rat hippocampus. Methods SpragueDawley rats aged 6 days were randomly divided into a recurrent-seizure group （RS） and a control group. At postnatal day 6 （ P6 ）, the recurrent seizures were induced by inhalation of the volatile agent flurothyl once a day for consecutive 6 days. The rats in the control group were placed in the container for an equal period of time as those in the RS group without exposure to flurothyl. Y-maze test was performed to evaluate learning and memory capacity at postnatal day 29 to 35 and 61 to 67, respectively. During the period of postnatal day 51 to 56, all the animals in the RS and control groups were subject to a 30-minute daily aerobic exercise program for consecutive 6 days. All the animals were sacrificed at postnatal day 78, and the in situ hybridization method was used to detect the expression of ZnT3 mRNA in hippocampus. Results ①The number of trials needed for getting correct response to the electric stimulation in the first Y-maze test was （60 ± 14.1 ） and （37.5 ± 17.2） for the RS and control groups, respectively （P 〈 0.05 ） , while that in the second Y-maze test carried out 24 hours later was （27.5 ±14.1 ） and （21 ± 11.01 ） for the RS and the control groups,respectively （P 〉 0.05 ）. ②Memory test revealed no significant difference between the RS and the control groups （ P 〉 0.05 ）. ③In situ hybridization detection showed that the expression of ZnT3 mRNA in hippocampus was not significantly different between the two groups. However, there showed a significant difference between the dentate gyrus and CA3 in the RS group with regard to the expression of ZnT3 mRNA （P 〈 0.05 ）. Conclusions Physical exercise improves the learning capacity of neonatal seizure-induced cognitive deficit and might have effects on the regulation of zinc transporter gene express