中文摘要：

目的：探究中空硅纳米粒在细胞线粒体内的定位。方法：以阿霉素为模型药物及荧光影像分子,通过激光共聚焦显微镜,考察中空硅纳米粒与线粒体分子探针在线粒体内的共定位。通过差速离心法提取线粒体,进一步确认硅纳米粒在线粒体内的聚集。结果：与线粒体分子探针为共定位参照,中孔硅纳米粒在线粒体中分布的皮尔逊系数为0.758±0.033,线粒体提取结果显示,进入细胞的纳米粒有90.01±3.89%分布于线粒体。结论：中空硅纳米粒具有自发聚集于线粒体的能力,可以作为药物靶向递送载体,为线粒体类疾病的治疗提供新的平台。

英文摘要：

Objective： To investigate the location of hollow silica nanoparticles in cells. Methods： Taken doxorubicin hydrochloride as the modal drug and fluorescence imaging molecular, the location of silica nanoparticles in cells were co-located with Mito-tracker Deep Red by laser scanning confocal microscope. The location of silica nanoparticles were further confirmed by isolation of mitochondrial and cytosolic extracts through differential centrifugation. Results： Compared with Mitotracker Deep Red, the Pearson’s Coefficient of the silica nanoparticles was 0.758±0.033, while the isolation of mitochondria showed 90.01±3.89% of the model drug distributed in mitochondria. Conclusions： The hollow silica nanoparticles can spontaneously gathered in mitochondria, which provides a new platform to mitochondria-targeted drug delivery system for mitochondria dysfunctional diseases.