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脉冲电磁场对C2C12成肌细胞增殖影响的实验研究

ISSN号：1673-6273
期刊名称：《现代生物医学进展》
时间：0
分类：Q64[生物学—生物物理学]
作者机构：[1]哈尔滨医科大学附属第四医院骨科,黑龙江哈尔滨150001, [2]哈尔滨市第一医院骨科,黑龙江哈尔滨150001
相关基金：黑龙江省卫生厅资助项目（2012-690,2009-174）; 哈市人才专项（2014RFXGJ041,2014RFQGJ094）; 黑龙江省科技计划攻关资助项目（GC10C303-4）; 卫生部医药卫生科技发展研究中心项目（W2012ZT30）

作者：李刚[1], 毕佳琦[2], 李卫[1], 曲志伟[2], 成勇[2], 李宝林[2], 林琛[2], 孟庆刚[1]

关键词：脉冲电磁场, C2C12成肌细胞, 增殖, Pulsed electromagnetic fields, C2C 12 myoblasts, Proliferation

中文摘要：

目的：研究不同强度脉冲电磁场干预对成肌细胞增殖的影响。方法：10Hz脉冲低频电磁场刺激经复苏后培养贴壁良好的C2C12成肌细胞,根据不同磁场强度和作用时间将其分为A、B、C组,无磁场干预的为对照组。采用RT-q PCR检测不同磁场强度下成肌细胞标记基因Myf5、Myo D及Pax7的m RNA的表达。结果：经RT-q PCR检测三种基因的表达情况,Myf5 m RNA在1.5 m T磁场强度下照射第五天表达最高;0.5 m T磁场强度下Myf5 m RNA的表达与对照组相比无统计学意义（P〉0.05）;1.0 m T磁场强度下Myf5 m RNA表达与对照组比较差异具有统计学意义（P〈0.05）;1.5 m T磁场强度下Myf5 m RNA表达与对照组比较差异具有统计学意义（P〈0.05）。对照组Myo D m RNA的表达要比磁场作用下表达要高。三个磁场强度下Myo D m RNA表达与对照组相比均无统计学意义（P〉0.05）。0.5 m T、1.0 m T磁场强度下Pax7 m RNA的表达要比对照组要高,与对照组相比具有统计学意义（P〈0.05）;1.5 m T磁场强度下Pax7 m RNA表达与对照组相比无统计学意义（P〉0.05）。结论：1.5 m T脉冲电磁场强度下作用5天对体外培养的成肌细胞Myf5 m RNA标记基因增殖促进作用最强。

英文摘要：

Objective： To study the effects of different intensity pulsed electromagnetic field interference on myoblast proliferation. Methods： Four groups were divided in the experimen： control group without intervention field; group A, in which the magnetic field strength was 0.5m T experimental, group B 1.0m T experimental, group C 1.5 m T experimental. Expression of marker gene Myf5, Myo D and Pax7 of m RNA was detected using RT-q PCR under different magnetic field strength myoblast. Results： By RT-q PCR detection of the expression of three genes, the expression of Myf5 m RNA was the highest on the fifth day of the irradiation field strength1.5m T; the expression of Myf5 m RA in the group magnetic field strength was 0.5 m T, which, compared with in the control group, was not statistically significant（P0.05）; the expression of Myf5 m RNA in the group magnetic field strength was 1.0 m T, compared with in the control group was statistically significant（P0.05）; the expression of Myf5 m RNA in the group magnetic field strength is 1.5 m T,compared with in the control group, it was statistically significant（P0.05）. The expression of Myo D m RNA in the control group was higher than the magnetic field. Under the three magnetic fields, strength Myo D m RNA expression, compared with in the control group,was not statistically significant（P0.05）. The expression of Pax7 m RNA in the group magnetic field strength was 0.5 m T and 1.0 m T,compared with that in the control group, it was statistically significant（P 0.05）; the expression of Pax7 m RNA in the group magnetic field strength was 1.5m T, compared with that in the control group, it was not statistically significant（P0.05）. Conclusions： 1.0 m T and1.5 m T pulsed electromagnetic fields on cultured myoblasts are with proliferation, but the growth promoting of cultured myoblasts marker gene Myf5 m RNA is the strongest at 10 Hz, 1.5 m T pulsed electromagnetic field strength.