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常春藤代谢气体甲醛中间产物及甲醛胁迫下叶片相关生理特性变化分析
  • 期刊名称:生科科学研究
  • 时间:0
  • 页码:-
  • 分类:Q946.92[生物学—植物学]
  • 作者机构:[1]昆明理工大学生命科学与技术学院生物工程技术研究中心,中国云南昆明650500
  • 相关基金:国家自然科学基金资助项目(30970263)
  • 相关项目:拟南芥一碳代谢网络对甲醛脱毒作用的机理研究
作者: 陈丽梅|
中文摘要:

很多研究发现常春藤(Hhelix)能吸收甲醛(HCHO),对常春藤气体HCHO吸收能力分析表明常春藤能有效清除空气中污染的气体甲醛.13CNMR分析发现用高浓度气体H13CHO处理常春藤,在叶片中出现的主要代谢中间产物为13CH3OH、[[5-13C]Met、U-13C]Gluc、[U-13C]Fruc、[3-13C]Ser、[2-13C]Gly、[5-13C]Arg、[3-13C]Ala、[4-13C]malate、[3-13C]malate及少量的[2-13C]Ser和H13C00H.此外,在气体H13CHO处理短时间的叶片抽提物中有游离H13CHO和甲醛加合物H13CHO—Gln,证实H13CHO确实被吸收到叶片细胞中,随着处理时间的增加这两个峰消失,说明通过自身的代谢作用H13CHO被有效清除.在气体甲醛胁迫下,常春藤叶片丙二醛、过氧化氢和羰基化蛋白质的含量升高,说明甲醛胁迫在常春藤叶片里诱发了氧化损伤,从而使叶片气孔传导率下降,甲醛吸收速率和弛率溅小

英文摘要:

Many studies have shown that Hedera helix (H.helix)could absorb formaldehyde (HCHO). The analysis of the capacity of H.helix to absorb gaseous HCHO showed that H.helix could remove air-polluted gaseous HCHO effectively. 13CNMR analysis indicated that, after treatment with high level of gaseous HCHO, the major intermediates produced from H13CHO metabolism in H.helix leaves included 13CHgOH, [5-13C]Met, [U-13C]Gluc, [U-13C]Fruc, [3-13C]Ser, [2-13C]Gly, [5-13C]Arg, [3-13C]Ala, [4-13C]malate, [3J3C]malate and a small amount of [2-13C]Ser and HI3COOH. Moreover, the presence of signal peaks for free H13CHO and H13CHO- Gln adduct in the short-traced leaf extract of gaseous H13CHO confirmed that H13CHO was indeed absorbed into leaf cells. With the treatment time increased, the two peaks disappeared, suggesting that H.helix could effectively eliminated H13CHO through metabolism. The content of malondialdehyde, hydrogen peroxide and earbonyl protein increased under gaseous HCHO stress, indicating that HCHO stress induced oxidative damage in H.helix leaves and thereby reduced leaf stomata conductance, HCHO uptake rate and efficiency.

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