目的:探索低频电刺激对中国树嗣血激素水平的影响。方法:给予中国树购低频电刺激或肌内注射利血平后再给予低频电刺激,用放射免疫法检测血清中去甲肾上腺素(NE)、内皮素(ET)、血栓素B:(TXB:)和睾酮(T)水平。结果:放射免疫法检测发现,给予中国树嗣低频电刺激后1h、24h、72h和7d,血清中T水平:电刺激组中24h比1h、72h和7d分别升高了12.49%、7.27%和15.79%,电刺激组比对照组显著升高(P〈0.01);利血平组中24h、72h和7d比1h分别降低了5.42%、11.80%和18.07%,利血平组比对照组明显降低(P〈0.05或P〈0.01)。血清TXB:水平:电刺激组和利血平组比对照组显著升高(P〈0.01),电刺激组间、利血平组间在1h、24h、72h和7d差异无统计学意义(P〉0.05)。血清ET水平:电刺激组中24h比1h、72h和7d分别升高了9.12%、11.41%和10.85%,电刺激组比对照组显著升高(P〈0.01);利血平组比对照组明显升高(P〈0.05),利血平组中1h、24h、72h和7d差异无统计学意义(P〉0.05)。血清NE水平:电刺激组中1h比24h、72h和7d分别升高了10.65%、5.83%和19.29%,电刺激组比对照组显著升高(P〈0.01);利血平组中24h、72h和7d比1h时分别降低了32.85%、43.38%和52.26%,利血平组比对照组明显降低(P〈0.05)。结论:低频电刺激能促进中国树胸血清NE、El'、TXB:和T释放。利血平可降低低频电刺激对机体的应激作用。
AIM: To study the low-frequency electric stimulation on the serum hormone levels in Chinese tree shrews. METHODS: Chinese tree shrews were given low-frequency electric stimulation, or intramuscularly injected with reserpine and then given low-frequency electric stimulation. The serum levels of norepinephrine (NE), endothelin (ET), thromboxane B2 (TXB2 ) and testosterone (T) were detected by radioimmunoassay. RESULTS: In electric stimulation group, the serum levels of NE, ET, TXB2 and T were significantly increased as compared with control group. After low-fre- quency electric stimulation, the serum level of T at 24 h was higher than those at 1 h, 72 h and 7 d by 12.49%, 7.27% and 15.79%, respectively. Reserpine treatment significantly reduced the T level as compared with control group, and the T level at 24 h, 72 h and 7 d after treated with reserpine was lower than that at 1st h by 5.42%, 11.80% and 5.42%, re- spectively. The levels of TXB~ in electric stimulation group and reserpine group were significantly higher than that in control group, and no difference in electric stimulation groups or in reserpine groups between 1 h, 24 h, 72 h and 7 d after electric stimulation was observed. The level of ET in electric stimulation group at 24 h after electric stimulation was higher than those at 1 h, 72 h and 7 d by 9.12%, 11.41% and 10.85%, respectively. The level of ET in reserpine group was signifi- candy higher than that in control group. However, no difference of the ET level in reserpine groups between 1 h, 24 h, 72 h and 7 d was observed. The level of NE after electric stimulation at 1 h was higher than those at 24 h, 72 h and 7 d by 10.65%, 5.83% and 19.29%, respectively. The levels of NE in reserpine groups at 24 h, 72 h and 7 d after electric stimulation were lower than that at 1st h by 32.85%, 43.38% and 32.85%, respectively. Compared with control group, the level of NE in reserpine group was significantly decreased. CONCLUSION: Low-frequency electric stimulation pro-motes the release