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基于电子回流理论的植物延迟荧光来源理论与实验研究
  • ISSN号:1007-7146
  • 期刊名称:《激光生物学报》
  • 时间:0
  • 分类:R318.51[医药卫生—生物医学工程;医药卫生—基础医学]
  • 作者机构:[1]哈尔滨工业大学机电工程学院,黑龙江哈尔滨150001, [2]大连海事大学信息工程学院,辽宁大连116026
  • 相关基金:The National Natural Science Foundation of China (60378043; 30470494) ; The Natural Science Foun- dation of Guangdong Province ( 015012 ;04010394 ).
中文摘要:

延迟荧光(DF)技术往植物学、农业和环境等领域有着广泛的应用前景,延迟荧光来源的机理研究对其应用起着重要的作用。基于广泛接受的电子回流坶论由一系列的光化学反应方程推导得出多指数衰减的延迟荧光动力学模型,首次提出了一个简化的三指数衰减近似模型,描述了光合电子传递链上的三个最重要成分的电子回流。为了验证近似模犁的准确性,以实验室培养的相似的8周大的Jin Dan No.39玉米叶片为模型样品,利用实验室自制的延迟荧光探测系统,得到延迟荧光衰减动力学曲线。分别用三种电子阻断剂:DCMU、DB—MIB和Paraquat处理后的衰减动力学的实验与理论模型进行了对比研究,通过曲线特征来确定不同成分的存在及依赖关系。结果表明延迟荧光衰减动力学三指数拟合非常好(R^2=0.99909),而且这三种成分是相互独立的,分别来源于QA,QR和光系统I环式电子流。简化模型的提出为延迟荧光的应用题提供一个平台。

英文摘要:

Delayed fluorescence (DF) technique has wide application foreground in the research of botany, agriculture, and environment and so on. The study of the mechanism for its origin plays an important role in the application of it. In the study, a multicomponents decay kinetics model of DF was deduced from a series of photochemical reaction equations based on the widely accepted theory of charge recombination. At the same time, a simplified three components approximation decay kinetic model was firstly proposed, which described the electronic reflux of three most significant components in the photosynthesis electron transport chain. To examine the accuracy of the approximation model, the labculture similar eight-week-old Jin Dan No. 39 leaves were adopted as the model sample to get the decay kinetics of DF using lab-made DF detection system. The comparison of theory model and experiments were accomplished in the presence of three electron blockers: DCMU, DBMIB, and Paraqaul. The results showed that the three components fit for decay kinetics of DF was very excellent (R2 =0. 99909). In addition, the three components had the independence and came from the election reflux of QA , QH and the cyelie electron flow around PS I , respectively. This representation of the simplified model provides a workbench for the application of DE.

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期刊信息
  • 《激光生物学报》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国遗传学会
  • 主编:胡能书
  • 地址:长沙市麓山路36号湖南师范大学生命科学学院内
  • 邮编:410081
  • 邮箱:jgswxb@hunnu.edu.cn
  • 电话:0731-8872208
  • 国际标准刊号:ISSN:1007-7146
  • 国内统一刊号:ISSN:43-1264/Q
  • 邮发代号:42-194
  • 获奖情况:
  • 2000年12月荣获首届《CAI-CD》执行优秀奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊
  • 被引量:5516