中文摘要：

目的探讨微小RNA（miRNA）-221和miRNA-222表达与胶质瘤病理分级及胶质瘤患者预后的关系。方法收集手术切除的胶质瘤标本120例，病理学分级I级19例、Ⅱ级31例、Ⅲ级38例、Ⅳ级32例；采用实时聚合酶链反应（Real—timePCR）及荧光原位杂交（FISH）法检测miRNA-221和miRNA-222的表达。对进行标准治疗的胶质瘤患者进行4年随访。结果肿瘤病理级别随miRNA-221和miRNA-222表达的升高而增加，组间比较差异有统计学意义（x2=43．53，P〈0．01）；生存时间随miRNA-221和miRNA-222表达升高而减少，各组间差异有统计学意义（x2=55．63，P〈0．01）。结论miRNA-221和miRNA-222的表达与胶质瘤的病理学分级及预后密切相关，可以作为独立的预后因素。

英文摘要：

Objective To investigate the relationship between the expression of microRNA （ miR- NA）-221 and miRNA-222 and pathological grade of glioma and prognosis of patients with glioma. Methods The expression of miRNA-221 and miRNA-222 was detected by real-tiem polymerase chain reaction （ Real- time PCR） and FISH in 120 cases of glioma. Of the 120 cases, 19 cases were fallen into pathological grade Ⅰ , 31 into grade Ⅱ , 38 into grade Ⅲ, and 32 into grade IV. The patients with glioma under the standard treatment were followed up for 4 years. Results Pathological grade of glioma was increased with the in- creased expression of miRNA-221 and miRNA-222, and there were significant differences between the groups （X2 = 43.53, P 〈 0. 01 ）. Survival time was decreased with the increased expression of miRNA-221 and miRNA-222, and there were significant differences between the groups （X2 = 55.63 ,P 〈 0. 01 ）. Con- clusion The expression of miRNA-221 and miRNA-222 was related to pathological grade and the progno- sis of glioma, and can be considered as the independent prognostic factor.