中文摘要：

目的：研究以HER2mRNA为靶点的反义硫代脱氧寡核苷酸（S—ODNs）HA6722单用及与榄香烯乳剂合用时对HER-2过表达乳腺癌细胞株MDA—MB-453体外增殖的抑制作用。方法：选择HER2过表达的MDA—MB-453细胞与HER2低表达的MDA—MB-231细胞，MTT法观察HA6722单用及与榄香烯乳剂合用时对两种肿瘤细胞增殖的影响。结果：HA6722及榄香烯单用均可以剂量依赖方式抑制MDA—MB-453细胞的体外增殖，IC50值分别为41．8±8．1 nmol·L^-1和79．4±12．1nmol·L^-1（n=3，mean±s）。加用低剂量的HA6722可增强榄香烯乳剂对MDA—MB-453细胞的抑制作用，IC50值由合用前的79．4±12．1nmol·L^-1降至51．6±8．2nmol·L^-1（n=3，P〈0．05）。相反，在HER2低表达的MDA—MB-231细胞则没有这种增强作用；在IC50浓度下二者之间的联合指数（CI）为0．72±0．38（n=3）。结论：反义寡核苷酸HA6722与榄香烯乳剂合用对HER-2过表达乳腺癌细胞的体外增殖抑制具有协同作用。

英文摘要：

Objective： To study the inhibitory effects of HER2 specific antisense oligodeoxynucleotide HA6722 administered alone or in combination with β-elemene on proliferation of breast cancer cell lines. Methods： MDA-MB453 and MDA-MB231 cell lines, which are HER2 over-and low-expressors, respectively, were used as experimental cells. Inhibitory effects of HA6722 alone or in combination with β-elemene on these cells were detected by means of methyl thiazolyl blue HA6722 and β-elemene could both inhibit the growth of MDA-MB453 cells in vitro in a dose-dependent manner with IC50 values of 41.8±8.1 nmol·L-1 and 79.4±12.1 nmol·L-1 （n=3）, respectively. When administered in combination with a low dose of HA6722, inhibitory effects of β-elemene on MDAMB453 cells increased markedly, resulting in significant decrease of IC50 from 79.4±12.1 nmol·L-1 to 51.6±8.2 nmol·L-1 （n= 3, P〈0.05）. The combination index （CI） was 0.72±0.38 （n=3）, which indicated a synergistic effect of these two drugs on HER2-overexpressing breast cancer cells. Conclusion： When administered in combination, antisense oligodeoxynucleotide HA6722 and β-elemene inhibit the growth of HER2-overexpressing breast cancer cells in a synergistic manner.