中文摘要：

目的 研究高致病性禽流感（HPAI）H5N1病毒NS1蛋白对干扰素诱导蛋白10（IP-10）的影响。方法 分别将禽流感病毒A／Anhui／1／2005（H5N1）的NS1基因、插入80～84位缺失氨基酸的NS1突变基因及流感病毒A／Puerto Rico／8／1934（H1N1）的NS1基因克隆至真核表达载体pEGFP-N1．转染人支气管上皮细胞BEAS-2B，流式细胞仪检测转染细胞内IP-10的表达情况。结果 与pEGFP-N1对照组相比，三种NS1蛋白均能下调BEAS-2B细胞IP-10的表达（P〈0．01），但三者之间下调程度差异无统计学意义（P〉0．01）。结论 A／Anhui／112005（H5N1）禽流感病毒单-NS1蛋白能够抑制BEAS-2B细胞IP-10表达，但这并不能完全阐明其与病毒致病性之间的关系。

英文摘要：

Objective To investigate the influence of avian influenza virus （AIV） NS1 protein on the expression of interferon-inducible protein 10 （IP-10）. Methods NS1 gene from virus A/Anhui/1/2005（H5N1 ）, NS1 gene inserted with 80-84 amino acids from virus A/Anhui/1/2005（ H5N1 ）and NS1 gene from virus A/Puerto Rico/8/1934（H1N1）were cloned into the eukaryotic expression vector pEGFP-N1, and transfected into BEAS-2B cells, IP-10 expression level in transfected cells was detected by flow cytometry. Results Compared with the control group pEGFP-N1, Expression of these three different NS1 genes can down-regulate the expression of IP-10 in BEAS-2B cells, but there is no significant difference as to the lower level among them. Conclusion NS1 protein of A/Anhui/1/2005 （H5N1） can down-regulate the expression level of IP-10, but this may not clarify its relationship with the virulence of AIV.