为了明确句容地区草莓枯萎病病原菌的种类和寻找草莓枯萎病的防治方法,2013年10-11月,分别对句容地区草莓枯萎病病菌进行了田间调查、分离培养、形态学观察描述、DNA序列分析、致病性试验和药剂田间防治试验。结果表明,分离到381块真菌菌落,镰刀菌属的真菌(Fusarium)占72.4%。通过形态特征、DNA测序和致病性试验确认草莓枯萎病病原菌为尖孢镰刀菌(Fusarium oxysoporum Schl.)。田间防治效果以250g/L吡唑醚菌酯EC2000倍液最好,灌根后30d的防治效果为100%,灌根后51d的防治效果为93.11%;其次是1%申嗪霉素SC 900倍液,灌根后30d的防治效果为100%,灌根后51d的防治效果为58.63%,其他参试药剂1×10^9CFU/g多黏芽孢杆菌(JX-13)WP1000倍液、1g含1×10^6个孢子的寡雄腐霉WP7000倍液、1g含6×10。有效活性菌数的哈茨木霉T-22GR500倍液等均有一定的防治效果。
A total of 381 fungal colonies were isolated from the strawberry fields infected with fusarium wilt in Jurong area, Jiangsu province, and cultured to identify the pathogen. Fusarium colonies accounted for 72. 4 percent. Morphologi-cal characteristics, DNA sequencing, and pathogenicity test revealed that the pathogen was Fusarium oxysoporum Schl. The field control effect was the strongest by 250 g/L pyraclostrobin EC 2 000-time solution, with the efficacies of 100. 00% after 30-day root application and 93. 11% even after 51-day root application, followed by phenazine-1-carboxylic acid SC 900-time solution with the concentration of 1%. Other fungicides such as paenibaccilus polymyxa, pythium oligandrum, and tri-choderma harzianum showed certain control effects on the disease as well.