中文摘要：

目的研究灵芝多糖（GIPS）抗肿瘤作用及其机制。方法通过相差显微镜和间接免疫荧光的方法鉴定了人脐静脉内皮细胞（HUVECs）。采用MTT法检测GIPS对人前列腺癌细胞（PC-3M）和HUVECs增殖的影响。检测G1PS对PC-3M细胞与HUVECs粘附的影响。采用transwell双层小室观察G1PS对PC-3M迁移穿过单层HUVECs的影响。结果GIPS对PC-3M无直接细胞毒作用，对HUVECs增殖无显著性作用。GIPS能够减少粘附和迁移穿过单层内皮细胞的肿瘤细胞数目。结论灵芝多糖通过抑制肿瘤细胞粘附并迁移穿过内皮细胞发挥其抗肿瘤作用。

英文摘要：

Aim To determine the possible mechanism of the anti-tumor effect of GIPS. Methods MTT assay was adopted to measure the proliferation of human prostate carcinoma （ PC-3M ） cells. Human umbilical vein endothelial cells （HUVECs） were cultured in vitro and identified by morphology and immunofluoreseence staining. Adhesion and migration ability of PC- 3M and HUVECs monolayer were examined. Results G1PS showed no cytotoxic effect on PC-3M cells as well as the proliferation of HUVECs. GIPS could signifi- cantly inhibit PC-3M adhesion to and migration through HUVECs. Conclusion G1PS exerts the anti-tumor activity via inhibition of PC-3M adhesion to and migration through HUVECs.