中文摘要：

目的：探讨细胞生长因子（GF）对生物电场（Ⅱ-诱导大鼠主动脉血管平滑肌细胞（VSMC）定向迁移的影响。方法：体外培养大鼠主动脉VCGMC，在不同条件培养基中用150mV·mm^-1 EF干预6h，显微成像及图象分析技术检测平滑肌细胞迁移情况。结果：在含有10％胎生血清（FBS）的培养基中，VSMC在EF作用下向阴极迁移。在无血清的培养基中，细胞没有定向迁移。在无血清培养基中补充血小扳源生长因子-BB、碱性成纤维细胞生长因子、转化生长因子-β1（PDGF-BB、bFGF、TGF-β1）时，部分恢复细胞向阴极迁移。结论：EF诱导的VSMC定向迁移依赖于血清的存在，血清中的生长因子与EF协同作用，促进细胞向阴极迁移。

英文摘要：

Objective：To investigate the effects of growth factor（GF） on directed migration of cultrued vascular smooth muscle cells（VSMC） among electrical field Methods：Cultured VSMC were exposed to physiological electrical field 150mV/min for 6 hours in the presence of DMEM,DMEM＋10% foctal bovine serum（FBS） or DMEM＋GF,theinterval photographs of VSMC were taken and analyzed.Results：In media containing 10% FBS,these cells migrated cathodally.The cells cultured in serum-free medium showed no directed migration.All of platelet derived growth factor-BB（PDGF-BB）,basic firbroblast growth factor（BFGF） and transforming growth factor-β1（TGF-β1） promoted VSMC directed migration in serum-free medium.Conclusion：Physiological electrical field induce VSMC directed migration.Some substances in serum are necessary to VSMC directed migration.including growth factors.