中文摘要：

目的制备功能化自组装多肽水凝胶并检测其与神经干细胞（NSCs）的生物相容性。方法合成自组装多肽RADA16与多肽RADA16-IKVAV，将两者混合制备功能化自组装多肽水凝胶，用原子力显微镜（AFM）观察其形态学特征。采用无血清培养法从新生大鼠皮层分离培养NSCs。将NSCs分别接种到RADA16自组装多肽水凝胶（对照组）与功能化自组装多肽水凝胶（实验组）表面。激光扫描共聚焦显微镜（LSCM）观察细胞迁移。用CCK-8法测定吸光度（A）检测细胞增殖能力。应用Nestin、MAP2、GFAP和CC-1免疫荧光染色，检测神经干细胞分化。结果AFM显示功能化自组装多肽水凝胶由纳米纤维组成，其纤维直径为20～30nm，长度可达数百纳米。在细胞接种6d后，对照组和实验组细胞在水凝胶中的迁移距离分别为（27．5±3．7）μm和（53．2±5．4）μm，两组差异有统计学意义（P〈0．05）。复合培养7d后，实验组中细胞A值明显高于对照组（P〈0．05）。免疫荧光结果显示13d后对照组中MAP2阳性细胞百分率为（22．44±3．52）％，实验组为（40．35±4．84）％，两组差异有统计学意义（P〈0．05）。结论功能化自组装多肽水凝胶与NSCs相容性良好。

英文摘要：

Objective To develop a functionalized self-assembling peptide hydrogel, and investi- gate its biocompatibility with neural stem cells （NSCs）. Methods The functionalized self-assembling peptide was made by mixing the self-assembling peptide RADA16 and peptide RADA16-IKVAV. The mor- phological features of the functionalized self-assembling peptide were studied by atom force microscope （AFM）. NSCs were isolated from the cerebral cortex of neonatal rats and cultured in serum-free medium. NSCs were seeded on the surface of both RADA16 self-assembling peptide hydrogel （control group） and the functionalized self-assembling peptide hydrogel （experimental group）. The proliferation behavior of the cells was evaluated by CCK-8 assay as expressed by absorbance value. Cell migration was detected by laser scan confocal microscope （LSCM）. Immunofluorescence staining with Nestin, MAP2, GFAP, and CC-1 was used to assess the differentiation of NSCs. Results AFM showed that the functionalized self-assembling peptide hydrogel was made up of nanofibers with diameter of 20-30 nm and length of hundreds nanometers. In 6 days after cell seeding, the depth of cell migration in experimental group [ （53.± 5.4） μm ] was significantly higher than that in control group [ （27.5 ± 3.7 ） μm, P 〈 0.05 ]. CCK-8 assay showed that experimental group displayed a significant increase of absorbance value compared with that of the control group after 1 -week culture （ P 〈 0. 05 ）. Immunofluorenscene analysis indicated that the differentiation ratio of neurons from NSCs in control group and experimental group were （22.44 ± 3.52） % and （40.35 ± 4.84）% respectively after 13-days culture. The difference between the two groups was significant （P 〈 0.05）. Conclusion The functionalized self-assembling peptide hydrogel has good cytocompatibility with NSCs.