中文摘要：

建立了美白类化妆品中熊果苷的两种光学异构体α-熊果苷和β-熊果苷及烟酰胺的高效液相色谱检测方法。样品用氯化钠水溶液-氯仿（2∶1,v/v）进行萃取。固定相为依利特ODS-BP柱（200mm×4.6mm,5μm）,流动相为甲醇-水（10∶90,v/v）,柱温为25℃,检测波长为220nm,流速为0.5mL/min,进样量为20μL。在上述条件下α-熊果苷、β-熊果苷和烟酰胺的质量浓度依次在0.07～50,0.06～50,0.05～50mg/L时与色谱峰面积之间的线性关系良好,相对标准偏差（n=7）分别为1.65%、1.73%和1.33%。将该方法用于化妆品的检测,回收率为91.7%～109.6%。该法简便、快速、准确,可用于化妆品美白成分的测定。

英文摘要：

A high performance liquid chromatography （HPLC） method for the determination of two optical isomers of arbutin （α-arbutin and β-arbutin） and niacinamide in cosmetics was developed. The samples were extracted by the mixture of salt water and chloroform （2∶1, v/v）. The separation was performed on an ODS-BP column （200 mm×4.6 mm, 5 μm, Elite） with methanol-water （10∶90, v/v） as the mobile phase at a flow rate of 0.5 mL/min and 25 ℃. The detection wavelength was set at 220 nm and the sample injection volume was 20 μL. There were good linear relationships between the mass concentration and the peak areas of α-arbutin, β-arbutin and niacinamide in the ranges of 0.07-50, 0.06-50 and 0.05-50 mg/L, respectively. The relative standard deviations （RSDs, n=7） of α-arbutin, β-arbutin and niacinamide were 1.65%, 1.73% and 1.33%, respectively. The proposed method has been applied for the determination of α-arbutin, β-arbutin and niacinamide in cosmetics with recoveries of 91.7%-109.6%. This method is rapid, simple and suitable for the detection of whitening ingredients in cosmetic.