中文摘要：

以小麦抗逆相关蛋白TaMAPK2作为诱饵，利用酵母双杂交系统进行筛库，获得互作蛋白TaAIP。氨基酸序列分析发现TaAIP具有wali保守区，并且与一些物种的铝诱导蛋白相似。实时荧光定量PCR分析显示，TaAIP基因受到铝、干旱以及高盐胁迫上调表达。半定量RT—PCR结果表明，TaAIP在小麦茎中表达，在根部、叶片以及花中没有表达。亚细胞定位试验发现，TaAIP定位在细胞膜上。这些结果为深入分析TaAIP的抗逆性作用机理奠定基础。

英文摘要：

TaAIP was obtained as the interaction protein of wheat stress-related TaMAPK2,which was used as bait protein to screen the wheat cDNA library by yeast-two-hybrid system. TaAIP, containing a similar to some aluminum-induced proteins. Real-time PCR showed that the expression of TaAIP by the imposition of aluminum, high-salt, and drought stress. Semi-quantitative RT-PCR analysis wali domain, was was up-regulated indicated TaAIP was a stem-specific gene,not expressed in root,leaves, and flower. The subcellular localization assay indicated that TaAIP located on plasma membrane. These results provided the foundation for further analysis of TaAIP resistance mechanisms.