中文摘要：

目的：探讨人转化生长因子TGF-β2特异性siRNA真核表达载体转染人结膜囊成纤维细胞后对其TGF-β2mRNA表达的影响。方法：体外分离并培养人结膜囊成纤维细胞,在培养后传代3次的细胞以TGF-β2特异性siRNA真核表达载体进行转染,并以未转染细胞作为对照。转染后分别于24,48和72h收集细胞,采用RT-PCR技术检测TGF-β2特异性siRNA真核表达载体对TGF-β2mRNA表达的影响。结果：分离的人结膜囊成纤维细胞于接种约4h左右开始贴壁,同时细胞变长成为梭形,表现出明显的成纤维细胞特性,约36h后达到融合状态;RT-PCR结果示：与对照组相比,转染24,48和72h后的细胞TGF-β2表达抑制率分别为17.40%,52.80%和79.20%,抑制效率呈现随时间延长有所加强的趋势。结论：TGF-β2特异性siRNA真核表达载体能抑制人结膜囊成纤维细胞TGF-β2mRNA的表达。

英文摘要：

AIM：To investigate the effects of siRNA for transforming growth factor β2（TGF-β2） on the expression of TGF-β2 mRNA in human Tenon＇s capsule fibroblasts.METHODS：Human Tenon＇ s fibroblasts were transferred by TGF-β2 siRNAs vector after separated,cultured and passaged three in vitro.RT-PCR was performed to evaluate the levels of TGF-β2 mRNA at 24,48 and 72 hours after transferred in transferred cells,and untransferred cells were as the controls.RESULTS：Separated human Tenon＇s fibroblasts attached in 4 hours and confluent monolayer cells formed in 36 hours.The adherent cells displayed fibroblast-like or spindle shape by microscope observation.The expression of TGF-β2 mRNA of transferred group was reduced significantly in comparison with the control group,expression inhibition rates were 17.40%,52.80% and 79.20% respectively in 24,48 and 72 hours after transferred,and this interference function was strengthened with the time extending.CONCLUSION：The vector of siRNA specific for TGF-β2 is of potent interference ability for TGF-β2 mRNA expression in human Tenon＇s capsule fibroblasts.