中文摘要：

目的探讨益肾活血胶囊提取液（YSHXC）对同型半胱氨酸（HCY）诱导的兔血管平滑肌细胞（VSMCs）转化生长因子β1（TGF-β1）表达的影响。方法组织贴块法体外原代培养兔VSMCs,由HCY诱导建立细胞增殖模型并分组：正常组、HCY刺激组（HCY1mmol/L）、HCY＋叶酸组（HCY1mmol/L＋叶酸5mmol/L）、HCY＋YSHXC大剂量组（HCY1mmol/L＋YSHXC5000mg/L）、HCY＋YSHXC小剂量组（HCY1mmol/L＋YSHXC500mg/L）。分别采用免疫组化及RT-PCR技术检测各组TGF-β1蛋白及mRNA的表达,同时通过MTT比色法测定细胞增殖活度。结果HCY刺激后细胞数目明显增多,细胞内TGF-β1表达显著增强（P〈0.01）,而YSHXC大、小剂量组细胞增殖活度减低,TGF-β1蛋白和mRNA的表达水平均下降（P〈0.01）,并呈现剂量依赖性（P〈0.05）,且此抑制作用优于叶酸组（P〈0.01或P〈0.05）。结论YSHXC可明显抑制HCY诱导的细胞内TGF-β1的表达,减少平滑肌细胞的增殖,这可能对延缓动脉粥样硬化进程起到一定的作用。

英文摘要：

Objective To explore the effect of extracting solution of capsule of tonifying kidney activating blood circulation （YSHXC） on TGF-β1 expression of vascular smooth muscle cells （VSMCs） in the rabbits induced by homocysteine （HCY）. Methods Primary VSMCs cultured by attachment-block in vitro were induced by HCY to establish cell multiplication model and divide into normal, HCY stimulation （HCY 1 mmol/L）, HCY ＋ folic acid （HCY 1 mmol/L ＋ folic acid 5 mmol/L）, HCY ＋ high dosage of YSHXC （HCY 1 mmol/L ＋ YSHXC 5 000 mg/L） ,HCY ＋ low dosage of YSHXC （HCY 1 mmol/L ＋ YSHXC 500 mg/L） groups. TGF-β1 protein and mRNA expressions were respectively detected by immunohistochemisty and RT-PCR. Activity of cell multiplication was determined by MTr colorimetric technique. Results After HCY stimulation, cell number obviously increased, TGF-β1 expression significantly enhanced （ P 〈 0. 01 ）, but high and low dosage of YSHXC groups had the decreased activity of cell multiplication ,the descended TGFβ1 protein and mRNA expressions （ P 〈 0. 01 ）, presenting dose dependent （ P 〈 0. 05 ）, the better effect than folic acid group （ P 〈 0. 01 orP 〈 0. 05 ）. Conclusions YSHXC could obviously inhibit TGF-β1 expressions induced by HCY,decrease VSMCs proliferation,which might be play a certain role in delaying atherosclerosis process.