中文摘要：

目的 探讨性别决定区Y框蛋白18（sex determining region Y-box 18,SOX18）在前列腺癌中的表达及其对细胞功能的影响.方法 通过免疫组化染色法检测SOX18在98例前列腺癌及其对应的81例正常组织中的表达差异;利用实时荧光定量PCR及蛋白印迹法验证SOX18在前列腺癌细胞系中的表达.利用小干扰RNA对前列腺癌细胞进行转染,通过细胞增殖、迁移及侵袭实验评估SOX18对细胞功能的影响.结果 在98例癌组织中,SOX18高表达阳性率为73.5％（72/98）,明显高于癌旁组织（43.2％,35/81） （P ＜0.01）.SOX18高表达的患者在临床分期Ⅲ ～ Ⅳ期、病理分级G3 ～4及Gleason评分≥8分者中所占比例明显高于临床分期Ⅰ～Ⅱ期[83.7％（36/43）与65.5％（36/55）,x2=4.131,P=0.042]、病理分级G1 ～2 [88.6％（39/44）与61.7％ （33/54）,X2=9.424,P=0.002]及Gleason评分≤7分者[85.0％（34/40）与65.0％（38/58）,x2=4.610,P=0.032],而不同年龄患者的SOX18高表达比例差异无统计学意义[77.3％（38/54）与70.4％（34/44）,x2=0.539,P =0.441].干扰前列腺癌细胞系（PC3和DU145）中SOX18的表达后,细胞的增殖、迁移和侵袭等能力明显降低,差异均有统计学意义（均P＜0.05）.结论 SOX18在前列腺癌中高表达,且促进前列腺癌细胞的增殖、迁移及侵袭等能力.

英文摘要：

Objective To explore the expression of sex determining region Y-box 18 （SOX18） in prostate cancer（PCa） and its role on prostate cancer cells.Methods The expression of SOX18 in 98 PCa tissues and 81 adjacent non-tumor tissues was detected by immunohistochemistry.The expression of SOX18 mRNA and protein in PCa cell lines were detected by q-PCR and Western blotting analysis,respectively.After knocking down SOX18 with si-RNA,the proliferation,migration and invasion of PCa cells were analyzed by CCK8,Transwell and Matrigel assays in vitro.Results The rate of high SOX18 expression in prostate cancer tissues was 73.5% （72/98）,which was higher than that in adjacent normal tissues （43.2%,35/81） （P 〈 0.01）.In addition,the rates of high SOX18 expression in clinical stage Ⅲ-Ⅳ [83.7 % （36/43） vs.65.5 % （36/55）,x2 =4.131,P =0.042],pathological grading G3-4 [88.6% （39/ 44） vs.61.7% （33/54）,x2 =9.424,P =0.002] and Gleason scores ≥ 8 [85.0% （34/40） vs.65.0% （38/58）,x2 =4.610,P =0.032] groups were higher than those in clinical stage Ⅲ-Ⅳ,pathological grading G1-2 and Gleason scores ≤7 groups.However,there were no differences in the rate of high SOX18 expression in different age groups [77.3% （38/54） vs.70.4% （34/44）,x2 =0.539,P =0.441].Knockdown of SOX18 notably suppressed the proliferation,migration and invasion of prostate cancer cells.Conclusions SOX18 is over-expressed in prostate cancer,and could promote the proliferation,migration and invasion ability of prostate cancer cells.