中文摘要：

水稻Os SSI2基因负调控水稻的抗病反应,为了研究该基因的抗性机理,构建p GEX-6P1-Os SSI2原核表达载体,转化大肠杆菌BL21细胞后利用IPTG诱导外源蛋白表达并优化表达条件,使用SDS-PAGE和Western Blot检测分析表达产物。结果表明：成功构建了p GEX-6P1-Os SSI2原核表达载体后进行原核表达,在IPTG浓度为0.3 mmol/L,温度为25℃,诱导表达7 h,通过SDS-PAGE和Western Blot检测发现Os SSI2蛋白的可溶性表达量最多,为进一步研究水稻Os SSI2蛋白的抗性机理奠定了基础。

英文摘要：

The rice OsSSI2 gene regulates negatively rice disease resistance. In order to dissect the resistance mechanism of OsSSI2, the recombinant prokaryotic expression vector pGEX-6P1-OsSSI2 was constructed and transformed into E.coli strain BL21. The OsSSI2 protein was induced with IPTG, and confirmed by SDS-PAGE and Western Blot analysis. The results revealed that the prokaryotic expression vector pGEX-6P1-OsSSI2 was constructed and expressed successfully. In the condition that IPTG concentration was 0.3 mmol/L, the temperature was 25 %, and the time induced expression was 7 h, maximum expression of OsSSI2 soluble protein was detected by SDS-PAGE and Western Blot, which laid the foundation for further research of resistance mechanism of OsSSI2 protein from rice.