中文摘要：

目的：探讨高住高练对高脂饮食肥胖大鼠肝脏SREBP-1C表达的影响。方法：出生21天离乳SD雄性大鼠280只．肥胖造模成功后挑选160只。2周适应性训练后筛选130只．随机分为13组：对照组（同时视为低住低练0周组、低氧安静0周组和高住高练0周组），低住低练1、2、3、4周组，低氧安静1、2、3、4周组，高住高练1、2、3、4周组。用水平动物跑台进行有氧耐力运动，低住低练各组在常氧下进行速度为26m／min、1h／d、6d／w的运动，分别持续1。4周；低氧安静各组每天在低氧下（氧浓度为13．6％，约相当于3500m海拔高度）生活，自由活动，不安排训练，分别持续1-4周；高住高练各组每天在低氧下（氧浓度为13．6％）生活23h，训练1h，速度为21m／min，6d／w，分别持续1-4周（非训练日生活24h）。对照组于正式实验开始前、安静组于相应周数后、运动组于最后一次训练后恢复24h取材。分别采用实时荧光定量PCR技术和WestemB10t技术检测大鼠肝脏SREBP-1c基因和蛋白表达。结果：相同训练模式不同周数之间比较，高住高练组大鼠肝脏SREBP-1cmRNA和蛋白表达在第1、2、4周较对照组均变化无统计学意义（P〉0．05）。第3周mRNA表达降低，与对照组比较差异有统计学意义（P〈0．05），第3周蛋白表达下降。与1、2周组之间的差异均有统计学意义（P〈0．05）。相同周数不同训练模式之间比较，第1、4周。高住高练组肝脏SREBP-1cmRNA和蛋白表达与低住低练组和低氧安静组比较差异无统计学意义（P〉0．05）．第2周高住高练组SREBP-1c蛋白表达高于低住低练组（P〈0．01）．第3周高住高练组SREBP-1cmRNA和蛋白表达低于低氧安静组（P〈0．05）。结论：3周高住高练抑制肥胖大鼠肝脏SREBP-1cmRNA和蛋白表达效果较好。

英文摘要：

Objective The purpose of this research is to investigate the effect of living high training high on SREBP-lc expression in obese rat liver. Methods One hundred and thirty 21-day-old SD male obese rats induced by high fat diet were divided into 13 groups randomly：0-week control group（same as 0-week living low training low group,0-week hypoxic control group,and 0-week living high traininghigh group） ; 1-, 2-, 3-, and 4-week living low training low groups ; 1-, 2-, 3-, and 4-week hypoxie control groups;and 1-,2-, 3-, and 4-week living high training high groups. Aerobic training of animals in living low training low groups were performed on a treadmill at the speed of 26 m/min with 0 slope, 1 hour per day,6 days per week,for 1,2,3,and 4 weeks respectively. Hypoxic control groups lived in a chamber with oxygen concentration of 13.6%（equivalent to 3500 m altitude） without training for 1,2,3, and 4 weeks respectively. Living high training high groups lived in hypoxic environment same as the control groups,23 hours per day,and exercised on the treadmill at 21 m/min, 1 hour each day,6 days per week, for 1,2,3, and 4 weeks respectively. The livers of rats in control group were removed at beginning of experiment, and in training groups at the corresponding week 24 hours after the last training. SREBP-lc mRNA expression and protein expression were measured respectively by real-time fluores- cence quantitative PCR and Western blot. Results As compared the different training durations in the same training mode,SREBP-lc mRNA and protein expressions only in 3-week living high training high group significantly lower than the control group, as well as than that in 1- and 2-week living high training high group （P 〈 0.05）. As compared the different training modes in the same training duration, SREBP-lc mRNA and protein expressions in 2-week living high training high group were higher than that in 2-week living low training low group （P 〈 0.01 ）;and in 3-week living high training high group were lower than that