中文摘要：

目的：探讨人参皂苷Rg1对衰老模型大鼠骨髓造血功能的影响及其机理。方法：SD大鼠随机分为4组,每组10只。衰老模型组,皮下注射D-半乳糖120mg/kg qd×42;Rg1衰老模型组,注射D-半乳糖剂量与时间同衰老模型组,第15d起腹腔注射Rg1 20mg/kg qd×28;正常对照组,皮下注射生理盐水qd×42;Rg1正常对照组,注射生理盐水qd×14,第15d起腹腔注射Rg1（同Rg1衰老模型组）。模型复制或药物注射完成后第2d,采外周血检测血白细胞总数与分类计数和晚期糖基化终产物（AGEs）,骨髓单个核细胞（BMNCs）计数,衰老相关β-半乳糖苷酶（SA-β-Gal）染色检测BMNCs衰老百分率,多向造血祖细胞集落（CFU-Mix）培养检测BMMCs形成集落能力;Western blotting检测BMMCs衰老相关蛋白P21和P53的变化;提取腹腔巨噬细胞进行培养,比色法检测巨噬细胞吞噬功能。结果：与衰老模型组比较,Rg1衰老模型组外周血白细胞数量增多,淋巴细胞比例升高,粒细胞比例降低,CD8＋T细胞所占比例降低,CD4＋T细胞所占比例升高,外周血AGEs水平降低,每根股骨的BMMCs细胞数增多,SA-β-Gal染色阳性的BMMCs明显降低,BMMCs形成CFU-Mix能力提高,P21和P53的表达下调,腹腔巨噬细胞吞噬中性红指数上升。结论：D-半乳糖复制的衰老模型大鼠骨髓造血功能损伤明显,人参皂苷Rg1对其致衰损伤有明确的保护作用,可能机理与调控p53/p21信号通路有关。

英文摘要：

Objective： To explore the effects and the relative mechanism of ginsenoside Rg1 on bone marrow hematopietic function in aging model rats,and provide theoretical and experimental evidences for searching effective natural medicine of antiaging or protecting hematopietic function. Methods： Male SD rats were randomly divided into normal group,Rg1 normal group,aging model group and Rg1 aging model group. Aging model group： the rats were administrated with D-galactose 120 mg / kg,qd × 42 by subcutaneous way; Rg1 aging group：the rats were also given D-galactose of the same dose and time as aging model group,and from the 15 th day on,rats were treated with Rg1 20 mg / kg qd × 28 by intraperitoneal way; Normal control group： The rats were injected with same volume saline qd × 42 by subcutaneous way; Rg1 normal group： the rats were given saline with the same volume,qd × 14,then received Rg1 20 mg / kg qd × 28 by same way. After 2 days of finishing the treatment,peripheral blood was collected to detect the number of leukocytes,analyzes classification of leukocyte and advanced glycosylation end products（ AGEs）; The femur was taken to count the number of bone marrow mononuclear cells（ BMMCs） in each femur,the proliferation of BMMCs was detected; Senescenceaccociated β-galactosidase（ SA-β-Gal） stain was analyzed aging BMMCs; multipotential hematopoietic progenitor（ CFU-Mix） was cultured to observe the colony formation of BMMCs; the expression of senescence-associated protein P21 and P53 in BMMCs was assayed by Western blotting; peritoneal macrophage was extracted and detected the phagocytosis of the macrophage by colorimetric method. Results： Compared with the aging model group,Rg1 aging model group can significantly increase the number of leukocytes,regulate the percentage of granulocyte and lymphocytes,the proportion of CD8＋T cells and CD4＋T cells; the number of BMMCs in each femur; decrease the ratio of SA-β-Gal positive BMMCs,the amounts of AGEs; enhance the ability of BMMCs to for