以发病大花黄牡丹为试材,采用常规法分离培养、形态学鉴定、致病性测定等方法确定病原,并通过平皿菌丝生长抑制法筛选有效防治药剂。结果表明:大花黄牡丹叶斑病的病原菌为半知菌亚门叶点霉属斑点叶点霉(Phyllosticta commonsii)。抑制该菌菌丝生长效果较好的杀菌剂是30%嘧霉·福美双可湿性粉剂和80%嘧霉胺水分散粒剂(500倍液),抑菌率分别为82%和84%。该研究可为病害的有效控制提供前期研究基础。
Taking disease Paeonialud lowii as test material,pathogen was identified by conventional cultivation,morphological identification,pathogenicity test,then effective medicament was screened by agar mycelial growth inhibition method.The results showed that the pathogen were fungi belonging to Deuteromycotina of Phyllosticta commonsii on leaf spot pathogen of Paeonia ludlowii.The 30%thiram wettable powder and 80% Pyrimethanil WDG(500times diluent liquid)had better effect to constrain its hypha growth in laboratory,the inhibitory rate were 82%and 84%,respectively.The results of this study provided a foundation for the effective control of the disease.