中文摘要：

目的：构建甜瓜抗枯萎病基因的植物表达载体，并将其转入新疆感病甜瓜。方法：含有目的片段pMD18-T/R-Fom-2经Sac I和Sal I双酶切后，回收目的片断，该片断连接到pCAMBIA1301-1上，得到R-Fom-2基因的植物表达载体pCA-Fom-2。通过冻融法将该载体导入根癌农杆菌LBA4404菌株中，并转化新疆感病甜瓜。结果：获得了具有潮霉素抗性的再生植株，经过PCR和RT-PCR鉴定结果表明，目的基因已经整合到甜瓜基因组中并初步表达。结论：通过粮癌表杆菌夼导法将外源基因成功导入甜瓜中。

英文摘要：

The expression vector of R - Fore - 2 gene was constructed and transformed into radon to get the transgenic plants.Method： The cloned vector pMD18 - T/R - Fom - 2 and expression vector pCAMBIA1301 - 1 were digested with restriction nuclease Sac I and Sol I. The large fragments were purified. The two large fragments of pMD18 - T/R - Fom - 2 and pCAMBIA1301 - 1 were ligated by T4 DNA ligase and the reactant was transferred into E. coli. The recombinant binary vector was transfered into Agrobacterium and infected melon. Result： After selection with antibiotic,the green plant showed positive for RT- PCR.The fragment of aimed gene was purified after enzymic digestion plasmid pMD18 - T/R- Fom - 2 with Sac I and Sal I and cloned into pCAMBIA1301 - 1 to obtain the recombinant plant expression vector pCA - Fom - 2.