中文摘要：

目的：探讨磁感应热疗联合放疗在小鼠4T1乳腺癌细胞株皮下移植瘤模型中的治疗作用。方法：小鼠经皮下移植4T1乳腺癌细胞株,建立皮下移植瘤模型,待肿瘤直径达到10mm左右,然后将其分为以下4组进行处理：标准对照组（未做任何特殊处理,C组）、单纯热疗组（48±2℃10min,MIH组）、单纯放疗组（10Gyx2次,R组）、热放联合组（10Gyx1次＋48±2℃10min,MIH＋R组）。观察各治疗组和对照组的肿瘤体积的大小、肿瘤体积抑制率。每组分别在实验处理后24小时处死小鼠应用免疫组化检测肿瘤组织中Bcl-2和Caspase-3的表达情况。结果：热疗后的第1-4周MIH＋R组的肿瘤体积抑制率分别为（64.38±17.97）%、（73.90±9.48）%、（77.43±10.71）%、（73.17±8.58）%;MIH组的分别为（40.66±21.46）%、（54.60±10.20）%、（51.22±5.05）%、（39.53±5.49）%;R组的肿瘤体积抑制率分别为（51.60±18.93）%、（57.53±9.76）%、（52.34±7.42）%、（35.42±4.14）%。与对照组相比,各治疗组均能抑制肿瘤的生长（P〈0.05）。其中以MIH＋R组抑瘤作用最显著。与其它组相比,Bcl-2在MIH＋R组的表达显著下降,而Caspase-3的表达显著上升,差异具有显著性（P〈0.05）。结论：磁感应热疗与放疗均能诱导肿瘤细胞凋亡,有效地抑制小鼠4T1乳腺癌细胞株皮下移植瘤的生长,而磁感应热疗与放疗的联合治疗的抑瘤作用最显著,而且降低了放疗剂量。

英文摘要：

Objective： To investigate the effects of magnetic induction hyperthermia and radiotherapy on subcutaneous tumor of 4T1 breast carcinoma in rats.Methods： After the induction of 4T1 breast carcinoma cells,the mices were randomly assigned to four groups： the control group（group C）,magnetic induction hyperthermia group 48±2℃ 10min,group MIH）,radiotherapy group（10Gy x2 fraction,group R）,combination group（10Gy x1 fraction ＋48±2℃ 10min,group MIH＋R）.The volume of the tumors were observed.24 hours after the treatment,3 mices of each group were killed and the tumor tissue were excised,the expression of the gene Bcl-2,Caspase-3 were determined with ABC immunohistochemical staining.Results： From the first week to the fourth week,the inhibitive rates of tumor volume of group MIH＋R was（64.38±17.97）%,（73.90±9.48）%,（77.43±10.71）%,（73.17±8.58）%,those of group MIH was（40.66±21.46）%,（54.60±10.20）%,（51.22±5.05）%,（39.53±5.49）% and those of group R was（51.60±18.93）%,（57.53±9.76）%,（52.34±7.42）%,（35.42±4.14）% respectively.Both magnetic induction hyperthermia and radiotherapy could inhibit the growth of tumor,but the therapeutic effect was more significant in group MIH＋R（P0.05）;The expression of Bcl-2 were significantly decreased and Caspase-3 were significantly increased in the combination groups（P0.05）.Conclusion： We found that both magnetic induction hyperthermia and radiotherapy could inhibit the growth of tumor,but the therapeutic effect was more significant in group MIH＋R（P0.05）.