位置：成果数据库 > 期刊 > 期刊详情页
Enhancement of the immobilization and discrimination of DNA probe on a biosensor using gold nanoparticles
  • ISSN号：1000-6818
  • 期刊名称：《物理化学学报》
  • 时间：0
  • 分类：R318.21[医药卫生—生物医学工程;医药卫生—基础医学]
  • 作者机构：同济医科大学环境医学研究所,武汉430030
  • 相关基金：国家自然科学基金资助项目!(No.39990570)
作者： Zhao Hongqiu, Lin Lin, Tang Ji’an, Duan Mingxing, Jiang Long
关键词： 壳聚糖, 敏感膜, 化学发光, 生物传感器
中文摘要：

以壳聚糖为膜材料制成薄膜，运用生物素－亲和素系统将辣报过氧化物酶（ＨＲＰ）固定在膜上，固定化过程对ＨＲＰ的活性无影响，而其稳定生提高，运用Ｌｕｍｉｎｏｌ－Ｈ２Ｏ２－ＨＲＰ化学发光体系，对水样中微量Ｈ２Ｏ２进行检测，在１０＾－６～１０＾－６ｍｏｌ／Ｌ范围内线性关系良好，回归方程：Ｙ＝２０．６４８Ｘ－２１５．２３９（Ｘ：ｎｍｏｌ／Ｌ），检测灵敏度为０．５ｎｍｏｌ／Ｌ。实验研制的壳聚糖膜可制成生物传感顺

同期刊论文项目
食管癌与肺癌易感个体预警的理论基础和方法
期刊论文 96 会议论文 10 获奖 4 著作 1
两亲分子有序组合功能体系研究
期刊论文 6
同项目期刊论文
细胞色素P4501A1基因变异与肺癌易患性（综述）
DNA biosenser with high sensitivity amplified by gold nanoparticles
食管癌表达上调基因全长cDNA的克隆及鉴定
Enhancement of the immobilization and discrimination of DNA probe on a biosensor using gold nanopart
基于BioMEMS技术的DNA芯片研究
基于MEMS技术的微反应器的设计和制作
SYBR Green染色法检测肺癌及癌旁组织端粒酶活性
A quantitative method to measure telomerase activity by bioluminescence connected with telomeric rep
基于MEMS技术的PCR生物芯片的研究
Cloning and characterization of EC45 gene which encoded human ribosomal protein L15 and overexpresse
微全分析系统中的MEMS技术
表面等离子体谐振（SPR）生化分析仪的研制
Identification of differentially expressed genes in esophageal cancer through SSH in combination wit
hOGG1基因与肿瘤（综述）
Fixation of single-stranded DNA nucleotide by self assembly technology
Study on colloidal Au-enhanced DNA sensing by quartz crystal microbalance
基质金属蛋白酶基因MMP20在人食管癌发生发展中的表达差异
DNA修复基因hOGG1多态与食管癌遗传易感性
Frequency of CYP2A6 gene deletion and its relation to risk of lung cancer and esophageal cancer in t
Impact of genetic polymorphisms in cytochrome P450 2E1 and glutathion S-transferases M1, T1, and P1
Segregation analysis of esophageal cancer in moderately high-incidence area of northern China
Gene expression profile changes in initiation and progression of squamous cell carcinoma of esophagu
RAI, one candidate gene associated with differentiation of human lung adenocarcinoma cells
Differential expression analysis of matrix metalloproteinase 20 (MMP20) in human esophageal cancer
基因传感器
Simultaneous determination of tramadol and lidocaine in urine by end-column capillary electrophoresi
Particle size effect of the DNA sensor amplified with gold nanoparticles
食管癌肿瘤标志物研究进展（综述）
Sensitivity enhancement of DNA sensors by nanogold surface modification
Genetic polymorphism in myeloperoxidase but not GSTM1 is associated with risk of lung squamous cell
Susceptibility to gastric cardia adenocarcinoma and genetic polymorphisms in methylenetertra- hydrof
苯并(a)芘对肺癌细胞DNA损伤及修复基因表达水平的影响
CYPIAI基因多态性与肺癌个体易感性研究
DNA损伤和修复的生物标记物研究（综述）
错配杂志交化学发光法检测细胞色素P450IAI基因多态性
Detection of CYPIAI polymorphisms with a colorimetric method based on mismatch hybridization
P53基因第72位密码子多态与肿瘤（综述）
DNA氧化损伤生物标记物8-OH-dG的检测及其在医学中的应用
表面等离子体谐振（SPR）生化分析仪的研制与应用
Determination of sulpiride by capillary electrophoresis with tris(2,2’-bipyridyl)ruthenium (II) elec
髓过氧化物酶基因?463G/A多态与中国人肺癌遗传易感性
Correlation between a single nucleotide polymorphism in the matrix metalloproteninase-2 promoter and
Bioluminescent method for detecting telomerase activity
利用生物发光法检测人群外周淋巴细胞DNA加合物水平
一种基于适配分子的偏振荧光检测法
食管癌早期癌变的研究和annexin I表达的意义（综述）
蛋白质组学在实体肿瘤标志物研究中的应用（综述）
食管鳞状上皮不典型增生中端粒酶逆转录酶的表达
A novel microgravimetric DNA sensor with high sensitivity
食管癌危险因素的meta分析
DNA切除修复标志物在肺癌、食管癌组织中的表达
纳米金颗粒在石英晶体微天平检测DNA中的表面修饰作用
顺铂对A549细胞DNA损伤修复生物标记物表达的影响
肺癌组织中DNA修复酶MGMT、DNA-PKcs和Ku的表达变化
髓过氧化物酶基因多态性与肺癌遗传易感性的研究
Sensitization to the cytotoxicity of cisplatin by transfecion with nucleotide excision repair gene X
利用表面等离子体谐振生物传感器实时监测酶促反应的一种新方法
叶酸与肿瘤（综述）
毛细管电泳电化学发光检测体系中正高压进样条件下正负离子电场放大进样效应
生物传感器的研究与发展
利用表面等离子体谐振技术检测金黄色葡萄球菌肠毒素B
SPR实验中的噪声因素及其补偿方法研究
Direct tris(2,2’-bipyridyl)ruthenium (II) electrochemiluminescence detection of polyamines separated
Determination of biogenic amines by capillary electrophoresis with pulsed amperometric detection
SPR生化分析仪对6-氧甲基鸟嘌呤-DNA甲基转移酶的检测
人尿嘧啶糖基化酶cDNA的克隆及其在食管鳞癌组织中的检测
Substantial reduction in risk of lung adenocarcinoma associated with genetic polymorphism in CYP2A13
The deregulation of arachidonic acid metabolism related genes in human esophageal squamous cell carc
Gene expression profiles at different stages of human esophageal squamous cell carcinoma
Cyclooxygenase-2 expression in squamous dysplasia and squamous cell carcinoma of the esophagus
Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous
Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamo
Miniaturized tris(2,2’-bipyridyl)ruthenium (II) electrochemiluminescence detection cell for capillar
食管癌患者血清CEA、SCC和Cyfra21-1含量检测及临床意义
Evaluation of sol-gel derived carbon composite electrode as amperometric detector for capillary elec
Determination of diphenhydramine by capillary electrophoresis with tris(2,2’-bipyridyl)ruthenium (II
Expression of cyclooxygenase-2(cox-2) in human esophageal cancer and in vitro inhibition by a specif
The enhancement effect of gold nanoparticles as a surface modifier on DNA sensor sensitivity
等倾干涉条纹在SPR现象中的规律研究
Chemiluminescent detection of genetic polymorphisms based on mismatch hybridization: application to
DNA修复基因XRCC1单核苷酸多态性与肺癌易感性
微芯片毛细管电泳电化学/电化学发光同时检测药物分子
Identification of differentially expressed genes in esophageal cancer through SSH in combination with high throughput reverse Northern screening
Differential expression analysis of matrix metallo- proteinase 20 (MMP20) in human esophageal cancer
O／W微乳中的2,4—二硝基氯苯水解反应
A study on organized assemblies in the sodium alkylcarboxylate/fatty alcohol/water systems
Location of ethanol in sodium dodecyl sulfate aggregates
Fluorescence quenching of anthracene by N,N-diethylaniline in the O/W microemulsion
Lamellar liquid crystal polymerization of sodium oleate/oleic acid/octadiene/water system
期刊信息
  • 《物理化学学报》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:北京大学化学与分子工程学院承办
  • 主编：刘忠范
  • 地址：北京大学化学楼
  • 邮编：100871
  • 邮箱：whxb@pku.edu.cn
  • 电话：010-62751724
  • 国际标准刊号：ISSN：1000-6818
  • 国内统一刊号：ISSN：11-1892/O6
  • 邮发代号:82-163
  • 获奖情况:
  • 中文核心期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘（网络版）,荷兰文摘与引文数据库,美国科学引文索引（扩展库）,英国科学文摘数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,英国英国皇家化学学会文摘,中国北大核心期刊（2000版）
  • 被引量:24781