中文摘要：

目的研究白三烯受体拮抗药孟鲁司特对哮喘小鼠肺组织中黏蛋白Muc5ac表达及支气管肺泡灌洗液中白细胞介素13（IL-13）分泌的影响，探讨孟鲁司特对哮喘气道炎症黏液高分泌影响的机制。方法18只清洁级BALB／c小鼠随机分为哮喘组、孟鲁司特组和正常对照组，每组6只。免疫组化法检测Muc5ac蛋白在小鼠肺组织的表达。收集支气管肺泡灌洗液（BALF），酶联免疫吸附测定（ELISA）法检测其中IL-13的水平。结果Muc5ac蛋白在哮喘小鼠肺组织中的表达为（0．572±0．028），较正常对照组（0．137±0．041）明显升高，孟鲁司特治疗组Muc5ac蛋白表达（0．497±0．054）较哮喘组明显降低。哮喘组的BALF中IL-13为（78．07±6．78）Pg·mL^-1，较正常对照组（28．58±12．98）Pg·mL^-1明显升高，盂鲁司特组为（51．2±3．53）Pg·mL^-1，较哮喘组明显降低。Muc5ac蛋白表达与BALF中IL-13水平呈直线正相关（r=0．861，p〈0．05）。结论孟鲁司特可能通过下调IL-13和Muc5ac蛋白的袁达．对哮喘气道黏液高分泌发挥治疗作用。

英文摘要：

Objective To study effects of leukotriene receptor antagonist montelukast on the expression of mucoprotein MucSac in lung and IL-13 secretion in bronchoalveolar lavage fluid （BALF） of asthmatic mice and investigate the possible mechanism. Methods Eighteen BALB/c mice were randomly divided into the control, asthmatic model and montelukast groups, 6 mice in each group. The expressions of Muc5ac protein in lung were detected by immunohistochemical assay. Interleukin-β level in BALF were analyzed via ELISA. Results The MucSac expression in asthmatic group （0. 572 ± 0. 028 ） was significantly upregnlated compared with control group （0. 137±0. 041, P 〈0. 01 ）. MucSac protein in the montelukast treated group （0. 497± 0. 054） was evidently lower than that of the asthmatic group（P〈0.05 ）. The level of IL-13 in BALF in the asthmatic group（78.07± 6.78） pg . mL^-1 was obviously increased compared with the control group（28.58±12.98） pg . mL^-1 ,P〈0.01, while, which in the montelukast group （51.2±3.53） pg . mL^-1 was distinctly lower than that of the asthmatic group （P〈0.01）. MucSac protein expression was positively correlated to the level of IL-β （r = 0.861,P〈0.05）. Conclusion Montelukast plays a therapeutic role in mucus hypersecretion of asthmatic airway, which is associated with down-regnlation of MucSac and IL-β.