以2个丹参(Salvia miltiorrhiza Bge.)品系杂交得到的94株F1代植株为作图群体,利用SSR(simple sequence repeat,简单重复序列)、SRAP(sequence-related amplified polymorphism,相关序列扩增多态性标记)和ISSR(inter-simple sequence repeat,内部简单重复序列)标记进行了首张丹参遗传连锁图谱的构建。该图谱包含8个连锁群,93个标记位点,覆盖长度400.1 cm,平均图距4.3 cm;各连锁群包括2~23个标记,遗传距离3.3~132cm。该图谱为丹参相关数量性状的基因定位、分离以及克隆奠定了重要基础。
The first genetic linkage map of Salvia miliorrhiza was constructed in 94 F1 individuals from an intraspecific cross by using simple sequence repeat (SSR), sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers. A total of 93 marker loci in the linkage map, consisting of 53 SSR, 38 SRAP and 2 ISSR locus were made up of eight linkage groups, covered a total length of 400.1 cm with an average distance of 4.3 cm per marker. The length of linkage groups varied from 3.3 -132 cm and each of them included 2-23 markers, separately. The result will provide important basis for QTL mapping, map-based cloning and association studies for commercially important traits in S.miltiorrhiza.