以蒸汽爆破预处理后的玉米芯为原料,进行了玉米芯酶解工艺条件的研究。粉碎后的玉米芯在压力2.8 MPa、保压时间240 s条件下蒸汽爆破预处理,在初始固形物含量为14%(w/v),pH 5.0的条件下,分别添加纤维素酶15 FPA/g(底物)、木聚糖酶225 IU/g(底物),同时添加环境因子MgSO40.005 g/g(底物)、Tween-800.001 g/g(底物),糖化48 h后,还原糖浓度达到71.81 g/L,糖化率达到80.85%。试验结果表明,蒸汽爆破预处理及添加适量环境因子对玉米芯的糖化效果影响显著。
In this study,Utilizing corn cobs after steam explosion as material to research on enzymatic hydrolysis of corn cobs.The results showed,maintenance pressure time 240 s and steam pressure 2.8 MPa,the initial ratio of solid content was 14%(W/V),added cellulase(15 FPA/g,zymolyte),xylanase(225 IU/g,zymolyte) under pH 5.0 respectively.Simultaneously the environmental factors(MgSO4 0.005 g/g and Tween-80 0.001 g/g) were added.After 48 hours,the final concentration of reducing sugars was 71.81 g/L and saccharification rate reached 80.85%.This study demonstrated that the steam explosion pretreatment and proper amount of environmental factors could significantly improve the reducing sugars of corn cobs.