中文摘要：

目的：探讨人参皂甙Rg1与钛微粒对大鼠颅骨成骨细胞的影响,为人工关节假体松动的防治提供新思路。方法：收集、纯化新生SD大鼠颅骨成骨细胞,筛选钛微粒,配制钛微粒浸提液,进行内毒素检测后,将培养的第3代成骨细胞以1×105个.mL-1的密度传代接种于5组培养液中,即正常组（含10%胎牛血清的DMEM培养基）、钛微粒组（体积比为0.1%的钛微粒混悬液＋含10%胎牛血清的DMEM培养基）、钛＋高Rg1组（体积比为0.1%的钛微粒混悬液＋终浓度为100μg.m-1的人参皂苷Rg1＋含10%胎牛血清的DMEM培养基）、钛＋中Rg1组（体积比为0.1%的钛微粒混悬液＋终浓度为50μg.m-1的人参皂苷Rg1＋含10%胎牛血清的DMEM培养基）、钛＋低Rg1组（体积比为0.1%的钛微粒混悬液＋终浓度为25μg.m-1的人参皂苷Rg1＋含10%胎牛血清的DMEM培养基）。连续培养24 h后,观察成骨细胞形态;采用酶联免疫吸附法检测细胞培养液中前列腺素E2、肿瘤坏死因子α、白细胞介素6、白细胞介素1及白细胞介素1受体拮抗剂的浓度;采用实时荧光定量法检测成骨细胞中环氧化酶2mRNA、肿瘤坏死因子αmRNA的表达;采用Western Blotting法检测成骨细胞中环氧化酶2蛋白的表达。结果：5组成骨细胞培养液前列腺素E2光密度值的差异有统计学意义（F=244.895,P=0.000）;钛微粒组、钛＋高Rg1组、钛＋中Rg1组均高于正常组[（53.362±0.307）,（41.048±0.431）,（38.998±0.234）,（31.687±0.466）,P=0.000,P=0.000,P=0.000];钛＋高Rg1组、钛＋中Rg1组、钛＋低Rg1组（32.501±0.124）均低于钛微粒组（P=0.000,P=0.000,P=0.000）;钛＋低Rg1组低于钛＋高Rg1组、钛＋中Rg1组（P=0.000,P=0.000）;钛＋高Rg1组高于钛＋中Rg1组（P=0.000）;正常组与钛＋低Rg1组比较,差异无统计学意义（P=0.168）。5组成骨细胞培养液肿瘤坏死因子α光密度值的差异有统计学意义（F=72.340,P=0.000）;钛微粒组、钛＋高Rg1

英文摘要：

Objective： To observe the effect of ginsenoside Rg1 and titanium（Ti） particles on rat cranioaural osteoblasts,so as to provide new preventive treatment of joint prosthesis loosening.Methods： The cranioaural osteoblasts were collected from the newborn SD rats and were purified.The Ti particles were screened and the leaching liquor of Ti were prepared.After the endotoxin detection,the third-generation osteoblasts were cultured in Dulbecco’s Modified Eagle Medium（DMEM） supplemented with 10% fetal bovine serum（normal group）,DMEM supplemented with 10% fetal bovine serum and 0.1%（volume ratio） Ti particles suspension（Ti particles group）,DMEM supplemented with 10% fetal bovine serum and 0.1% Ti particles suspension and Ginsenoside Rg1 with final concentration of 100 μg / mL（Ti ＋ high Rg1 group）,DMEM supplemented with 10% fetal bovine serum and 0.1% Ti particles suspension and Ginsenoside Rg1 with final concentration of 50 μg / mL（Ti ＋ middle Rg1 group）,DMEM supplemented with 10% fetal bovine serum and 0.1% Ti particles suspension and Ginsenoside Rg1 with final concentration of 25 μg / mL（Ti ＋ low Rg1 group）,one hundred thousand osteoblasts in one milliliter of culture solutions.The osteoblasts were cultured for 24 hours continuously and then the shape of them were observed.The concentration of prostaglandin E2（PGE-2）,tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,interleukin-1（IL-1） and interleukin 1 receptor antagonist protein（IL-1ra） in the culture solution were detected through enzyme-linked immunoadsordent assay（ELISA）.The expression of cyclooxygenase 2（COX-2） mRNA and TNF-α mRNA in the osteoblasts were detected through real-time fluorescence quantitative polymerase chain reaction,and the expression of COX-2 protein in the osteoblasts were detected through Western Blotting.Results： There were statistical differences in the optical density（OD） values of PGE-2 among the 5 groups（F = 244.895,P = 0.000）.The OD values of PGE-2 wer