采用培养板浸液法和三角瓶药膜法研究了黄蒿挥发油对蚊虫幼虫和成虫的毒杀效果,采用GCMS-DS联用分析了其化学成分.结果表明,挥发油对淡色库蚊幼虫和成虫具有较高的作用效果,且作用速度较快.对幼虫处理后2d的LC50值约为12.5mg·L^-1.综合毒力测定显示,挥发油对成虫具有明显的击倒和致死作用,1μg·cm^-2和10μg·cm^-2剂量24h致死率分别为70%和100%.黄蒿含挥发油0.38%,共鉴定出38种化合物,检出物占总质量87.53%,其中萜烯类12种,占45.04%,为黄蒿挥发油的主要成分.
The study showed that Artemisia scoparia contained 0.38% of volatile oil, in which, a total of 38 chemical components were identified, accounting for 87.53% of the substances detected, and 12 kinds of terpenoids compounds were the main components, accounting for 45.04% of the total. The oil had a high and rapid poison activity on Culex pipiens pallens larva and adult. The LC50 value for the larva was 12.5 mg·L^-1 within 2 days, and the mortality of the adult in 24 hours was 70% and 100% when the dosage was 1 and 10 μg·cm^-2.