中文摘要：

利用同源序列从蒙古冰草（Agropyron mongolicum Keng）克隆得到1个光合作用叶绿体结合a/b基因，命名为MwLhcb1。MwLhcb1基因cDNA全长1138bp，包含801bp开放阅读框，编码267个氨基酸，蛋白分子量为28.21kD，等电点4.92。该蛋白二级结构中具有Lhcb基因家族的保守结构域。MwLhcb1蛋白氨基酸序列与其他物种同类蛋白相似性均在87%以上，其中与小麦同类蛋白相似性程度最高达99%。实时荧光定量PCR结果显示，MwLhcb1基因主要在茎叶中表达，在根中表达量极少,干旱胁迫影响MwLhcb1基因表达。该研究结果为进一步研究MwLHcb1在蒙古冰草光合作用与抗旱性中的功能奠定了基础，并从基因遗传进化角度证实了蒙古冰草是小麦野生近缘种的观点，从而提出蒙古冰草是小麦抗性改良的理想基因供体。

英文摘要：

The gene of light-harvesting cholorophyll a/b-binding proteins was cloned by homologous sequences from Agropyron mongolicum Keng,named MzvLhcbl.The full-length of cDNA sequence was1138bp,bioinformatics analysis showed that MvuLhcbl had an open reading frame of801bp,encoding267amino acids.The calculated molecular weight of MwLhcbl was28.21kD and isoelectric point was4.92.The MwLhcbl protein had a conserved domain of Lhcb gene family in the secondary structure.Phylogenetic analysis revealed that MwLhcbl amino acids sequences was highly similar with the homologous proteins of other species,it was more than87%,and the similarity degree of protein with wheat was the highest,it is99%.Real-time quantitative PCR analysis showed that MzvLhcbl gene was mainly expressed in stem and leaf,and the expression in the root was minimal.The expression of MzvLhcbl gene was affected by drought stress,and the expression of MwLhcbl gene was different under the different levels of drought stress.The results could be a foundation for further study on the function of MwLhcbl gene in photosyn thesis and drought resistance of A.mongolicum.Meanwhile,it confirmed was wild relatives of wheat based on gene genetic and evolution,so we supported that was ideal donor for improving to wheat resistance.