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中文摘要：
[目的]克隆溶藻弧菌（Vibrio alginolyticus）喹诺酮类耐药基因qnr并分析其蛋白结构，为研究该蛋白的生物学功能奠定基础。[方法]根据NCBI上公布的相关序列设计qnr基因特异性引物，利用PCR方法扩增基因序列，进行DNA测序及生物信息学分析。[结果]从溶藻弧菌染色体上获得qnr基因大小为651bp，编码216个氨基酸，进化分析可知其与副溶血弧菌有很近的亲缘关系，二级结构分析含有五肽重复序列，三维结构与大肠杆菌质粒上的qnr蛋白空间结构极为相似。[结论]通过对蛋白质序列分析和结构预测，初步确定该基因为喹诺酮耐药基因，为水产细菌的耐药机制研究奠定基础。
英文摘要：
[ Objective ] Quinolone resistance gene of Vibrio alginolyticus was cloned and the structure of the protein was predic- ted. It will provide the foundation for further study of the function of qnr. [ Methods] The quinolone resistance gene （qnr） was amplified by PCR and then was analyzed by bioinformatics methods after DNA sequencing. [ Results ] The sequencing analysis of qnr gene fragment showed that it was 651 bp, encoding a protein of 216 amino acids which had high homology with that of Vibrio parahaemolyticus. The secondary structure contained pentapeptide repeats, and three - dimensional structure of the pro- tein was very similar to qnr gene of plasmid in E. coll. [ Conclusion] By sequences analysis and structure predictions of qnr pro- tein,the gene was initially identified as a quinolone resistance gene, and laid the foundation for aquatic bacterial resistance mechanisms.
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