中文摘要：

基于渤海表层典型的碳酸盐系统,通过实验室密闭培养实验,分析急性CO2酸化条件对菲律宾蛤仔（Ruditapes philippinarum）CaCO3形成速率（G）和CO2呼吸速率（RC）的影响,以探讨局部海域CO2酸化的底层海水在潮流或者风海流等因素的驱动下,脉冲式影响贝类栖息地时养殖贝类可能的响应。结果表明,菲律宾蛤仔在急性CO2酸化条件下发生轻微的钙壳溶解和显著的呼吸抑制。CO2酸化和菲律宾蛤仔的呼吸作用共同驱动钙壳溶解,溶解速率随Ω文石下降而升高,G（μmol/（FWg·h））=0.14×Ω文石-0.49（n=12,r=0.95,p〈0.01）。活体菲律宾蛤仔钙壳保持稳定的Ω文石临界值为3.5,而在Ω文石=1.0的条件下,每天溶解的钙壳相当于贝壳总重的2‰。相较于钙壳溶解,Ω文石改变对菲律宾蛤仔呼吸作用的影响更大,RC（μmol/（FWg·h））=0.27×Ω文石＋0.90（n=12,r=0.82,p〈0.01）。由于呼吸代谢决定了摄食等各种耗能行为的效率,因此本研究的结果表明,尽管菲律宾蛤仔可以通过摄食等自身调节机制来抵御造成钙壳溶解的环境胁迫,然而这一机制本身就可能受到酸化环境的不良影响。

英文摘要：

A popularly cultured marine shellfish（Ruditapes philippinarum）was exposed to a series of CO2-acidified seawaters for 4hours.The waters had a constant salinity of 30.7and an alkalinity of 2 350μmol/kg.Both were the typical values of the surface water of Bohai Sea.Using the alkalinity anomaly method,effects of CO2 driven ocean acidification on the calcification and respiration of R.philippinarum were investigated.The results showed that,slight shell dissolution and significant respiration decline of R.philippinarumoccurred under several short-term CO2-acidified scenarios.A function of CaCO3 production rate（G）versus CaCO3 saturation of aragonite（Ωarag）had been developed as G（μmol/FWg·h）=0.14× Ωarag-0.49（n=12,r=0.95,p〈0.01）.This function suggested that the shell is instable on the conditions ofΩarag 3.5.However in a scenario whereΩarag decreases to 1.0-1.5in CO2-acidified subsurface waters in the Bohai Sea（in late summer）and the North Yellow Sea（in autumn）,the daily dissolved shell is only equivalent to insignificantly2‰ of the net shell weight.In contrast,respiration declines and metabolic activity changes of R.philippinarumunder CO2-driven acidification were more notable.The function of inorganic carbon production rate（RC）of R.philippinarumversusΩarag was RC（μmol/FWg·h）=0.27×Ωarag ＋0.90（n=12,r=0.82,p〈0.01）,suggesting that declines ofΩarag had negative impacts on respiration much more than shell dissolution.