中文摘要：

[目的]观察三七总皂苷（PNS）对大鼠骨髓间充质干细胞（MSCs）增殖和体外诱导其分化为心肌样细胞的作用。[方法]采用骨髓细胞体外培养技术分离大鼠MSCs，以流式细胞仪检测细胞表面标志；采用四甲基偶氮唑盐（MTT）法观察PNS对MSCs增殖的影响；采用第8代MSCs进行体外心肌细胞诱导分化；采用相差显微镜、免疫组化及逆转录聚合酶链反应（RT—PCR）结果鉴定心肌细胞。[结果]大鼠MSCs细胞表面抗原CD44阳性，CD34阴性；经PNS作用后MSCs增长速度比空白对照组明显加快，体外诱导后细胞形态发生变化，其细胞免疫组化鉴定呈结蛋白（Desmin）、肌钙蛋白（cTnI）阳性；RT—PCR结果显示诱导后细胞转录肌球蛋白重链mRNA水平增加。[结论]PNS能促进大鼠MSCs体外增殖并可诱导MSCs分化为心肌样细胞，这为细胞移植治疗心肌梗死提供了实验依据。

英文摘要：

[ Objective ] To observe the in-vitro effect of panax notoginseng saponins （PNS） on bone marrow mesenchymal stem cells （MSCs） proliferation and on inducing MSCs to differentiate into cardiomyogenic cells. [ Methods ] MSCs were isolated from adult SD rats, and then were cultured and cloned by density gradient method and adhesive cultivation. The cell surface markers of MSCs were detected with flow cytometer. The effect of PNS on MSCs proliferation was observed with methyl thiazolyl tetrazolium （MTT） assay. The 8th generation of continuous cultured MSCs was used to observe the in-vitro differentiation of cardiomyogenic cells. The cardiomyogenic cells were identified under phase contrast microscope by immunohistochemical method and reverse transcription- polymerase chain reaction （RT-PCR） analysis. [ Results ] CD44 expression of MSCs was positive while that of CD34 expression of bone marrow hematopoietic stem cells was negative, indicating the success of the MSCs culture. After induction with PNS, the increase of MSCs was obvious as compared with the blank control group, and there existed histological changes of MSCs after in-vitro induction. The expression of specific proteins of Desmin and cardiac troponin I （cTnI） in MSCs was positive, and the results of RT-PCR analysis showed that the expression of myosin heavy chain （MHC） was increased. [ Conclusion ] PNS can increase the in-vitro proliferation of MSCs and induce MSCs to differentiate into cardiomyogenic cells in vitro, and this will supply experimental evidence of cell transplantation for the treatment of myocardial infarction.