采用SSR分子标记分析了云南松主分布区范围内20个天然群体的遗传多样性、遗传分化以及与生态因子间的相关性。结果表明:云南松天然群体SSR遗传多样性指标与地理、气象和土壤因子间存在一定相关关系,表现为低纬度、低海拔、温暖、降水量多的环境下遗传多样性丰富;Mantel检测群体遗传距离的地理变异方式较弱,遗传变异不存在明显的地理隔离,与环境因子间的关系也未达到显著水平,但生态隔离的作用明显高于地理隔离的作用,表明地理、气候和土壤因子对云南松群体间的遗传分化有一定的影响,其中以气候因子对云南松遗传多样性的影响最大。
The genetic diversity and genetic differentiation of 20 Pinus yunnanensis Franch. natural populations collected from the main distribution areas were studied by Simple Sequence Repeat (SSR) marker. The relationships among the genetic diversity and the main ecological factors, i.e., geographical, climatic and soil parameters, were analyzed. The genetic diversity parameters based on SSR loci for 20 natural populations were correlated with geographical, climatic and soil parameters. Those populations with rich genetic diversity existed in low latitude, low altitude, warm and moist environment. There was no significant relationship between matrices of genetic distance and matrices of geographical distance by Mantel test, which indicated that there was no evident sign of geographical isolation. But the ecological isolation was more obvious than geographical isolation. The genetic differentiation among populations may be influenced by geographical, climatic or soil factors. Climate factor had a main influence on genetic diversity of P. yunnanensis.