中文摘要：

【目的】利用分子标记技术对鸭茅杂交种进行快速鉴定和遗传分析,建立鸭茅杂交种的快速鉴定体系,揭示鸭茅杂交种群体遗传信息,为鸭茅资源保护利用及新品种选育提供科学依据。【方法】利用SRAP标记技术对140个鸭茅杂交种单株及其亲本（01996、YA02-103）DNA进行扩增,分析杂交种与其亲本间扩增谱带的多态性,以鉴别真假杂交种,并结合形态标记对杂交后代的遗传变异进行分析。【结果】从192对SRAP引物中筛选出64对引物在杂交种和双亲间存在扩增多态性,多态性百分比为33.33%。利用能扩增出父本特征带的引物鉴定了140个杂交种,其中106个具有父本的特征带,可鉴定为真杂交种。选择16对多态性引物对亲本和106个真杂交种进行扩增,获得151条条带,其中多态性条带71条,平均每对引物扩增出4条多态性条带,多态性位点百分比为47.24%。【结论】SRAP用于鸭茅杂交种鉴定及多态性分析是可行的。

英文摘要：

【Objective】 Molecular markers were used to identify orchardgrass hybrids and analyze genetic diversity for forming orchardgrass hybrid identification system,detecting genetic information and conservation and utilization of orchardgrass germplasm resources. 【Method】 The sequence-related amplified polymorphism （SRAP） were assessed to analyze 140 orchardgrass hybrid individuals and their parental lines （01996,YA02-103） for detecting true or false hybrids according to polymorphism,and the genetic variation among hybrid individuals were detected using molecular markers and morphological markers. 【Result】 Sixty-four pairs of primers that generated clear,reproducible and polymorphic bands were selected from 192 pairs of SRAP primers with an average of 33.33%. 106 progenies with the special male bands compared with the female bands were identified from 140 progenies,which were therefore recognized as true hybrids. Sixteen primer pairs produced a total of 151 bands,of which 71 bands were polymorphic bands and the average polymorphic bands was four per primer pair. The average percentage of polymorphic bands was 47.24%. 【Conclusion】 These results suggested that SRAP technique has wide prospective in hybrid identification and diversity analysis of orchardgrass.