中文摘要：

目的通过检测原发性肝细胞癌中F10基因的表达，探讨该基因对肝细胞癌发生和发展的可能作用。方法采用地高辛标记F10探针，原位杂交检测人肝细胞癌组织及癌旁组织F10基因的表达水平；通过实时荧光定量PCR方法，在PEABI 7000型荧光定量PCR仪上检测肝细胞癌患者手术切除的癌组织及癌旁组织F10基因拷贝数。结果原位杂交结果表明肝癌组织F10基因阳性表达，癌旁组织F10基因不表达；荧光定量PCR显示8例肝细胞癌标本中有6例肿瘤组织中F10基因表达明显高于癌旁组织，其中2例高出4倍以上。结论原发性肝癌组织中存在F10基因的高表达，相应的癌旁组织中未见其表达，提示F10基因在肝细胞癌的发生、发展中起一定的作用。

英文摘要：

Objective To study the expression of a virulent novel gene F10 associated with hydatidiform mole in the hepatoma carcinoma cells （HC/2）, and to explore the effects of F10 on the occurrence and development of primary hepatocellular carcinoma （PHC）. Methods The full-length cDNA sequence of F10 was obtained from the samples of early pregnant chorionic villi and hydatidiform mole by employing suppression subtractive hybridization and cDNA microarray techniques. The hepatocarcinoma tissues and their surroundings were examined by in situ hybridization with digoxigenin-labeled of F10 gene. The real-time-fluorescence quantitative polymerase chain reaction （FQ-PCR） was used to quantitatively detect the expression level of F10 gene in primary hepatocellular carcinoma. F10 gene RNA copies in the samples were examined in the hepatocarcinoma tissues samples and their surroundings with PHC by PE ABI 7000 Sequence Detector. Results F10 mRNA was detected in hepatocarcinoma cells with positive signals found in kytoplasm and cell membrane, but not detected in the tissues surrounded the hepatocarcinoma. In 6 of 8 PHC samples, the expression Level of F10 gene was higher in tumor tissues than that in the tissues surrounded the tumor tissue. Among them 2 cases showed more than 4 times over-expression of F10 gene in tumor cells than in the surrounded tissues. Conclusion The virulent new F10 gene associated with hydatidiform mole was mainly expressed in hepatocellular carcinoma cells. The results of present study suggested that the higher expression of F10 gene not only showed a close relation to trophoblastic tumor, but also indicated that the F10 gene may play an important role in the occurrence and development of primary hepatocellular carcinoma.