东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

新奇 decellularizing 的评估为设计应用的角膜织物的角膜的基质

ISSN号：2222-3959
期刊名称：《国际眼科杂志：英文版》
时间：0
分类：R318.1[医药卫生—生物医学工程;医药卫生—基础医学]
作者机构：[1]Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China, [2]Department of Endocrinology, the Third Hospital of Nanchang, the Affiliated Nanchang Hospital of Southern Medical University, Nanchang 330009, Jiangxi Province, China, [3]Department of Physiology, Endocrinology Institute of Oklahoma University, Oklahoma City, OK 73104-5020, USA, [4]Department of Ophthalmology, the First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China
相关基金：National Natural Science Foundation of China (No.81160118,81100648,81101858,81100649);Natural Science Foundation of Jiangxi Province,China (No.20114BAB215029);Technology Foundation of Jiangxi Province,China (No.20111BBG70026-2);Health Department Science and Technology Foundation,China (No.20121026);Education Department Youth Scientific Research Foundation,China (No.JJJ12158);National High Technology Research of China (863 project)(No.2006AA02A131)

作者： Yi Shao,[1] Yao Yu,[2] Chong-Gang Pei,[1] Qiong Zhou,[1] Qiu-Ping Liu,[3] Gang Tan,[4] Jing-Ming Li,[3] Gui-Ping Gao,[1], Lu Yang[1]

关键词： CORNEA, human, SERA, ELECTROPHORESIS, tissue, engineering, DECELLULARIZATION, BIOCOMPATIBILITY, cornea, human sera, electrophoresis, tissue engineering, decellularization, biocompatibility

中文摘要：

·AIM:To develop a new decellularization method depended upon the natural corneal structure and to harvest an ideal scaffold with good biocompatibilities for corneal reconstruction.METHODS:The acellular cornea matrix (ACM) were prepared from de-epithelium fresh porcine corneas (DFPCs) by incubation with 100% fresh human sera and additional electrophoresis at 4℃. Human corneal epithelial cells (HCEs) were used for the cytotoxicity tests of ACM. ACM were implanted into the Enhanced Green Fluorecence Protein (eGFP) transgenic mouse anterior chamber for evaluation of histocompatibility.RESULTS:HE and GSIB4 results showed fresh porcine cornea matrix with 100% human sera and electrophoresis could entirely decellularize stromal cell without reducing its transparency. ACM has no cytotoxic effect ex vivo. Animal test showed there was no rejection for one month after surgery.CONCLUSION:These results provide a decellularizing approach for the study of corneal tissue engineering and had the broader implications for the field of biological tissue engineering in other engineered organ or tissue matrix.