中文摘要：

目的：研究TLRs与NLRs协同促进树突状细胞成熟的效应。方法：TLRs的配体与NLRs的配体刺激小鼠DC2.4细胞24h后用流式细胞仪检测细胞表面标记CD40、CD83、CD86、CD80与MHC-Ⅱ分子的表达。结果：单一TLRs或NLRs的配体可促进DCs表面标记分子表达的升高,并且2种配体的同时刺激可使以上标记分子的表达高于单一配体刺激。结论：TLRs与NLRs可协同促进DCs的成熟。

英文摘要：

Objective：To study the effect of TLRs and NLRs on the maturation of dendritic cells.Methods：The DC2.4cells were stimulated with a single ultrapure TLR/NLR ligand or a combination of two ligands.After 24 hours,the flow cytometry was used to test the expression of CD40,CD83,CD86,CD80,and MHC-Ⅱon the surface of DCs.Results：A single TLR or NLR ligand could promote the expression of DCs surface markers,and the combined stimulation of two ligands could enhance the expression of the above markers.Conclusion：TLRs and NLRs can synergistically promote the maturation of DCs.