中文摘要：

目的观察氯雷他定对支气管肺泡灌洗液（BALF）和血清IL-5表达以及嗜酸性粒细胞（EOS）计数的影响,探讨氯雷他定对哮喘豚鼠模型呼吸道高反应性的作用。方法将32只豚鼠随机分为正常对照组、哮喘组、氯雷他定低剂量组及氯雷他定高剂量组。经鸡卵白蛋白（OVA）致敏后,给予OVA雾化吸入,诱发哮喘发作制作哮喘模型,氯雷他定低剂量组和氯雷他定高剂量组分别给予氯雷他定2 mg.kg-1和10 mg.kg-1灌服。在末次雾化吸入OVA后,经动物呼吸机测定豚鼠呼吸道压力,用酶联免疫法检测其血清和BALF中IL-5水平,并计数外周血及BALF中EOS。结果正常对照组、哮喘组、氯雷他定低剂量干预组及高剂量干预组血清IL-5水平分别为（0.27±0.04）ng.L-1、（0.41±0.03）ng.L-1、（0.38±0.02）ng.L-1及（0.31±0.03）ng.L-1,BALF中IL-5水平分别为（0.10±0.01）ng.L-1、（0.38±0.04）ng.L-1、（0.33±0.05）ng.L-1及（0.23±0.09）ng.L-1;外周血EOS计数分别为（4.0±0.6）×108L-1、（54.6±7.6）×108L-1、（44.5±3.9）×108L-1及（29.0±5.8）×108L-1;BALF中EOS计数分别为（3.0±0.5）×108L-1、（33.7±3.9）×108L-1、（27.1±3.2）×108L-1及（18.6±3.3）×108L-1。以上各指标任意2组比较差异均有统计学意义（Pa〈0.05,0.01）。当吸入组胺质量浓度为10 mg.L-1和20 mg.L-1时,哮喘组与氯雷他定低剂量组比较差异无统计学意义,其余任意2组比较差异均有统计学意义（Pa〈0.05）。结论氯雷他定低、高剂量均能改善哮喘模型中豚鼠呼吸道高反应性,并对血清及BALF中IL-5的表达有抑制作用,同时使血清、BALF中EOS计数减少。

英文摘要：

Objective To observe the influence of loratadine which was the second generation of H1 receptor antagonist on the expressions of interleukin 5（IL-5）and eosinophil（EOS） count in bronchoalveolar lavage fluid（BALF） and peripheral blood in asthmatic guinea pigs,and explore the role of loratadine on airway high response of asthmatic guinea pigs. Methods Thirty-two guinea pigs were divided into normal control group,asthmatic group,low dose and high dose group of loratadine.After ovalbumin irritated,ovalbumin was inhaled to induce asthma breakout,meanwhile giving loratadine 2 mg·kg-1,10 mg·kg-1 in low dose group and high dose group.After the last inhaling ovalbumin,the airway pressure was detected by the animal respirator.Enzyme linked immunosorbent assay was used to measure the IL-5 in BALF and peri-pheral blood,meanwhile,EOS count was also detected. Results The serum IL-5 in normal control group,asthmatic group,low dose group and high dose group were（0.27±0.04） ng·L-1,（0.41±0.03） ng·L-1,（0.38±0.02） ng·L-1 and（0.31±0.03） ng·L-1;IL-5 in BALF were（0.10±0.01） ng·L-1,（0.38±0.04） ng·L-1,（0.33±0.05） ng·L-1 and（0.23±0.09） ng·L-1,respectively;the count of EOS in serum were（4.0±0.6）×108L-1,（54.6±7.6）×108L-1,（44.5±3.9）×108L-1 and（29.0±5.8）×108L-1;EOS in BALF in each group were（3.0±0.5）×108L-1,（33.7±3.9）×108L-1,（27.1±3.2） ×108L-1 and（18.6±3.3） ×108L-1.The differences between every 2 groups in each measurements were significant（Pa0.05,0.01）.When inhaled histamine 10 mg·L-1 and 20 mg·L-1,the differences between asthmatic group and low dose of loratadine group was not remarkable,but the difference between the rest 2 groups were prominent（Pa0.05）. Conclusions In asthmatic model,loratadine can ameliorate the airway high response,reverse the high IL-5 expressing condition in asthma,at the same time,the count of eosionphil is also decreased in both the BALF and blood.