中文摘要：

目的：检测骨形成蛋白（BMP）受体抑制剂LDN-193189对人去分化软骨肉瘤（DDCS）细胞系NDCS-1的抑制作用,探讨LDN-193189对去分化软骨肉瘤的抑癌机制。方法：以5 nmol/L的LDN-193189作用于NDCS-1细胞,MTT、平板克隆法检测LDN-193189对NDCS-1细胞的增殖抑制作用,Transwell法、划痕实验检测LDN-193189对NDCS-1细胞的侵袭抑制作用,Western blot检测BMPR2、p-Smad1/5及RUNX2的蛋白表达抑制情况。结果：药物处理后NDCS-1细胞增殖、侵袭被明显抑制;药物处理后NDCS-1细胞的BMPR2、p-Smad1/5及RUNX2蛋白表达下降。结论：LDN-193189通过抑制BMPR2-p-Smad1/5-RUNX2信号传导通路能有效抑制去分化软骨肉瘤细胞系NDCS-1的增殖侵袭能力。

英文摘要：

Objective：To clarify the effects of the BMP receptor inhibitor LDN-193189 in the dedifferentiated chondrosarcoma （DDCS） cell line NDCS-1 and to explore the anti-tumor mechanism of LDN- 193189 in DDCS. Methods： NDCS-1 was treated with 5 nmol/L of LDN- 193189 . MTT assay and clone formation experiments were used to verify that LDN- 193189 suppressed cell proliferation. Transwell and wound healing tests were performed to demonstrate that LDN-193189 inhibited cell invasion. Western blot detection was used to show that LDN- 193189 inhibited the suppression of BMPR 2, p-Smad 1/5, and RUNX 2 protein expression. Results：The BMPR2 signaling pathway was inhibited by LDN- 193189 ; thus, cell viability and invasion were significantly suppressed. Conclusion： LDN- 193189 induces the inhibition of progression in vitro via the BMPR2-p-Smad 1/5-RUNX 2 signaling pathway in the human DDCS cell line NDCS- 1.