中文摘要：

利用丝网印刷技术制备了基于Ru（bpy）3^2＋（钉联吡啶）的印刷电极电致化学发光传感器，这种传感器具有制作简单、成本低、重现性好、对草酸盐的响应范围宽、检测限低等优点。详细研究了电极的制作方法以及发光试剂的固定化。在最优条件下，在pH6．0的0．2mol·L^-1磷酸盐缓冲液中，利用所研制的ECL传感器测定C2O3^2-，线性响应范围为3．0×10^-7～1．0×0^-5mol·L^-1，检测限为1．2×10^-7mol·L^-1（S／N=3）。根据同样的原理也可以用来测定其他的成分，如氨基酸，TprA（三丙胺），NAD（烟酰胺腺嘌呤二核苷酸）等物质。同时应该指出的是如果使用丝网印刷机器进行印刷的话，电极的重现性和稳定性还可以进一步提高。

英文摘要：

The present paper reports the establishment of an electrochemi-luminescence （ECL） sensor by using screen-printed electrodes（SPE） which contain Ru（bpy）3^2＋ . The method of making the SPE and the fix of Ru（bpy）3^2＋ were studiedin detail. The characters of the sensor were studied. The sensor has been applied to detect C2O4^2- in solution. Under optimised cond：.tions, at 1.55 V vs Ag/AgCl, in 0. 2 mol·L^-1 phosphate buffer solution （pH 6.0）, the linger range extends from 3.0×10^-7mol· L^-1 to 1.0X 10^-5 mol·L^-1 , the detection limit is 1.2×10^-7 mol· L^-1 （S/N=3）. The sensors have good stability and reproductivity. It should be noted that based on the same principle, the sensors can be applied to detect many other compounds, such as amino acids, TprA and NAD. If the screen machine is used to make the SPE, the reproductivity and stability of the SPEs can be improved further.