中文摘要：

稀土农用的环境安全问题是限制稀土在农业上应用的关键。实验以辣根为研究材料，采用生态毒理学中的急性毒性试验方法研究不同生长阶段与不同温度条件下Tb（Ⅲ）对HRP的生态毒性。结果表明，比较不同生长阶段不同温度下对照CK的HRP活性，数据显示不同生长阶段HRP活性对温度响应不尽一致。辣根在不同生长阶段和不同温度下，HRP对高、低剂量Th（Ⅲ）毒害的响应存在明显差异。进一步比较不同温度各生长阶段的半效应剂量（EC50）及Tb（Ⅲ）对HRP酶活的最小作用剂量（LOEC）、最大无作用剂量（NOEC）变化规律，不同生长阶段温度对Th（Ⅲ）的生态毒性的影响不同（9月份影响最小），5、9月份HRP对低温敏感，7、11月份HRP对高温敏感，各阶段适温下HRP对Tb（Ⅲ）胁迫的敏感性小于低、高温。

英文摘要：

The environmental safe of rare earth used in agriculture is the key problem to limit rare earth application on agriculture. Horseradish was used as an experimental material to study ecotoxicity of Terbium at different temperatures during different growing periods by determining its acute toxicity. The results showed that effects of temperature on the activities of HRP were different in different growing periods by comparison of the activities of HRP of control （CK）. There was a significant difference between the response of HRP to high and low concentration of Tb（ Ⅲ ）under different temperatures during different growing periods. The effects of temperature on Terbium ecotoxicity varied remarkably with growing periods（the minimum effect appeared in September）by the comparison of 24 h-EC50 of Tb（ Ⅲ ）and LOEC and NOEC at different temperatures during different growing periods. Among all the growing periods May and September were sensitive to low temperature while July and December were sensitive to high temperature, but all periods were less sensitive to natural temperature.