中文摘要：

建立了一种非探针标记型磷酸化多肽的电化学检测方法,该方法以N,N-二（羧甲基）-L-赖氨酸（Lys-NTA）和Fe3＋修饰的金电极作为磷酸化多肽的捕获和传感界面,利用磷酸化多肽能够与之形成金属离子螯合物而发生特异性结合并引起电极表面修饰层通透性改变等特点,采用铁氰化钾作为电化学指示剂,用循环伏安法和交流阻抗法对磷酸化多肽的结合进行表征和检测。磷酸化多肽浓度在5～75μmol/L时,循环伏安图中峰电流随着磷酸化多肽浓度的增大而增大,而非磷酸化多肽不能引起峰电流变化,表明该电极体系能够特异识别磷酸化多肽,并实现灵敏快速的检测。

英文摘要：

A label-free electrochemical method for the detection of phosphorylated peptides has been established by using N,N-bis（carboxymethyl）-L-lysine（Lys-NTA） and Fe3＋ co-modified Au electrode as a capture and sensing interface.Phosphorylated peptides can specifically bind to the modified electrode through the formation of an ion chelate compound,which would lead to the change in the impedance signal of electrode surface.Thus cyclic voltammetry and electrochemical impedance spectrum can be used to characterize and determine phosphorylated peptides by using [Fe（CN）6]3-/[Fe（CN）6]4-as electrochemical probe.In the range of 5～75 μmol/L,the peak current in cyclic voltammogram increases with the elevated concentration of phosphorylated peptides.Whereas non-phosphorylated peptides could not bind to the electrode and would not lead to any changes in the electrochemical signal.Therefore,the proposed method can distinguish phosphorylated peptides specifically,and achieve sensitive and rapid detection.