中文摘要：

采用荧光光谱（FS）、核磁共振（NMR）、电喷雾离子化质谱（ESI-MS）和透射电镜（TEM）方法研究过氧钒配合物（NH4）[VO（O2）2（bipy）]·4H2O（1）和（NH4）[VO（O2）2（phen）]·2H2O（2）与朊蛋白淀粉样肽Pr P106-126的突变体M109F肽的相互作用。结果表明,过氧钒配合物可直接与M109F肽结合,并通过甲硫氨酸112的氧化作用来达到对M109F肽聚集的抑制作用。与配合物2比较,配合物1显示出对M109F肽更好的抑制作用;过氧钒配合物1和2都可以有效地降低M109F肽诱导的细胞毒性。本工作为潜在金属药物用于神经退行性疾病的研究提供了基础数据。

英文摘要：

Interactions of peroxovanadium complexes（NH4）[VO（O2）2（bipy）]·4H2O（1） and（NH4）[VO（O2）2（phen）]·2H2O（2） with the mutant peptide M109 F of prion neuropeptide Pr P106- 126 have been investigated by fluorescence spectroscopy（FS）, nuclear magnetic resonance（NMR）, electron spray ionization mass spectrometry（ESI- MS） and transmission electron microscopy（TEM） methods. The results show that the peroxovanadium complexes may directly bind to the M109 F peptide, and react with the peptide by methionine oxidation at Met112, resulting in the inhibition of M109 F peptide aggregation. Compared with complex 2, complex1 shows improved inhibitory effects on peptide M109 F. Both complexes 1 and 2 exhibit an increased cellular viability against amyloid peptide- induced cytotoxicity. The basic data for the investigation of potential metallodrugs against neurodegenerative disease are provided.