采用室内生物活性测定法,研究了中药羌活粗提物减轻乙草胺对水稻幼苗伤害的效果和对水稻幼苗谷胱甘肽S转移酶(GSTs)活性的影响。结果表明:在乙草胺质量浓度为0.05 mg/L的条件下,当羌活粗提物质量浓度分别为0.050 0、0.125 0、0.250 0、1.250 0 g/L时,水稻幼苗的株高分别恢复到清水对照的77.9%、95.1%、96.1%、96.2%;株鲜重恢复到清水对照的80.9%、98.8%、94.0%、98.4%;GSTs活性检测表明,羌活粗提物能诱导水稻幼苗地上部GSTs活性增强,当羌活粗提物的质量浓度为0.150 0、0.200 0和0.250 0 g/L时,水稻幼苗地上部GSTs的活性分别为清水对照的1.5、2.0和2.1倍。
Indoor bioassay was carded out to study the effects of crude extract of Rhizoma et Radix Notopterygii on reducing the phytotoxicity of acetochlor to the rice shoots, and on induction of GSTs of rice shoots. The results showed that under the of acetochlor concentration of 0.05 mg/L, the plant height restored to 77.9%, 95.1%, 96.1%, 96.2% of those in the control, and the plant weight restored to 80.9%, 98.8%, 94.0%, 98.4% of those in the control, when mass concentrations of extract from Rhizoma et Radix Notopterygii were 0.05, 0.125, 0.25 and 1.25 g/L, respectively. GSTs activity in the above-ground shoots of rice can be increased by the induction of extract from Rhizoma et Radix Notopterygii, when the concentrations of the extract were 0.15 g/L, 0.20 g/L and 0.25 g/L, the GSTs activity in the above-ground shoots was respectively 1.5-, 2.0- and 2.1-fold higher than that of the control.