中文摘要：

以前，我们成功地 poly 准备了(vinyldiaminotriazine )(PVDT ) 基于经由与腺嘌胸腺嘧啶结合的氢的原生质标志 DNA complexed 基于对的非病毒的向量。在这份报告，表面费用和这个系统的复杂尺寸进一步被检验。结果证明那基于 PVDT 的聚合物能盖住导致稍微否定或中立的建筑群的 DNA 的表面费用。复杂尺寸被二个事件管理，这也被发现：聚集由中立粒子的不稳定性导致了，并且更紧缩的建筑群由基于 PVDT 的聚合物生产了。在细胞的举起的学习，在 III 的氯普马嗪和弹指被用来分别地禁止调停 clathrin 、调停 caveolae 的 endocytosis。我们发现基于 PVDT 的系统经由 non-clathrin 被搬运进房间， non-caveolae 调停了 endocytosis。这个特殊过程被温度抑制和动力学试金学习。如此的一条小径被(i) 描绘，这被揭示一个更多的精力依赖者过程并且(i i ) 慢 transfection 有效的成为主观。

英文摘要：

Previously we successfully prepared poly（vinyldiaminotriazine）（PVDT）-based non-viral vectors which complexed piasmid DNA via hydrogen bonding with adenine-thymine base pairs, in this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events： the aggregation induced by the instability of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin III were used to inhibit clathrin-and caveolae-mediated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by （i） a more en- ergy dependent process and （ii） a much slow transfection-effective internalization.