中文摘要：

对原花青素的提取及测定进行了系统研究。考察了料液比、乙醇浓度、提取温度、提取时间等因素对原花青素提取的影响，并通过正交实验确立了最适合的提取条件为：料液比为1：6、乙醇浓度为60％、提取温度50℃、提取时间60min。极差分析表明提取温度对原花青素提取影响最大，其次是乙醇浓度、提取时间和料液比。铁盐催化比色法系统考察了反应时间、反应温度、反应介质的种类、酸的种类、酸的浓度、金属离子的种类及Fe^3＋的含量等因素对吸光度的影响，确立最适测定条件为：以正丁醇及5mL的浓盐酸的混合液作为反应介质，浓度0．415mol／L的Fe^3＋作为催化剂，60℃下反应60min，测得的吸光度最大。

英文摘要：

Extraction and determination of procyanidins from the grape seed were systematically studied. Effects of solid-to-liquid ratio, extraction agent concentration, extraction temperature, extraction time on extraction of procyanidins were investigated, and by orthogonal experiment the optimum extraction conditions were determined as follows： solid-to-liquid ratio 1：6, ethanol concentration 70%, extraction temperature 50 ℃, extraction time 60 min. The range analysis showed that the extraction temperature was major factor, the secondary factors were ethanol concentration, extraction temperature and solid-to-liquid ratio. The content of proanthocyanidins was determined by colormetry with ferric ion as catalyst. Effects of reaction time, reaction temperature, reaction medium, acid type, acid concentration, ferric ion concentration and metal ion type on the absorbency were researched. Through experimental analysis, the optimum conditions were determined as follows： using n-butanol and 5 mL hydrochloric acid as reaction medium ,0.415 mol/L Fe3＋ as a catalyst, reaction time 60 min, reaction temperature 60℃ ; under above conditions, the absorbency is the maximum.