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Cd胁迫下超富集植物圆锥南芥抗氧化机理
  • 期刊名称:环境科学学报
  • 时间:0
  • 页码:409-414
  • 分类:X171.5[环境科学与工程—环境科学] Q946[生物学—植物学]
  • 作者机构:[1]中山大学环境科学与工程学院,广州510275, [2]广东省环境污染控制与修复技术重点实验室,广州510275, [3]广西师范大学环境与资源学院,桂林541004
  • 相关基金:国家高技术研究发展计划(863)项目(No.2007AA06Z305, 2007AA061001);国家自然科学基金-广东联合基金重点项目(U0833004);广东省自然科学基金团队项目(06202438);广西教育厅项目(200707MS048)
  • 相关项目:华南矿山及周边地区多金属污染土壤联合修复技术与机理研究
中文摘要:

采用土培的方法研究了不同Cd^2+添加量(0、10、20、40、80、160、240mg.kg^-1)对多金属超富集植物圆锥南芥(Arabis paniculata Franch)抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶POD、抗坏血酸过氧化物酶CAT、过氧化物酶APX)活性、非酶物质(巯基SH、谷胱甘肽GSH、植物络合素PCs)含量以及生物量、叶绿素、丙二醛(MDA)、半胱氨酸(Cysteine)、脯氨酸(Proline)含量的影响.结果表明,随着Cd添加量的增加,圆锥南芥的生物量、Cd含量、富集系数、转运系数呈增加趋势,SOD活性、叶绿素含量呈下降趋势.POD、CAT、APX活性以及MDA含量均呈先升高后降低的变化趋势.圆锥南芥叶片中SH、GSH、PCs含量随Cd添加量的增加而增加,当Cd添加量为240mg.kg^-1时,SH、GSH、PCs含量分别比对照提高了92.7%、70.2%、143.4%,表明SH、GSH、PCs在解毒Cd毒害中起着重要作用.

英文摘要:

The effects of cadmium (Cd) on various enzymatic antioxidants and non-enzymatic antioxidants in Arabis paniculata Franch were studied.A.paniculata,a newly identified multi-metal hyperaccumulator,was grown in soils with various Cd concentrations (0,10,20,40,80,160 and 240 mg·kg^-1) for 135 days.Plant leaves were analyzed for contents of glutathione (GSH),phytochelatins (PCs) and total acid soluble SH,chlorophyll,malondialdehyde (MDA),cysteine,proline and activities of superoxide dismutase (SOD),peroxidase (POD),catalase (CAT),and ascorbate peroxidase (APX).The results showed that the plant biomass,Cd content,bioaccumulation factor and translocation factor increased,while the chlorophyll content and SOD activities decreased as the soil Cd concentrations rose.The activities of enzymatic antioxidants (POD,CAT and APX) and the contents of MDA increased at soil Cd concentration below 40 mg·kg^-1and then decreased after this.The contents of GSH,PCs and SH rose with Cd concentration.Compared to the control,the contents of GSH,PCs and SH increased by 92.7%,70.2% and 143.4% respectively at a soil Cd concentration of 240 mg·kg^-1.The results indicated that both enzymatic and non-enzymatic antioxidants play significant roles in Cd detoxification in this plant.

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