中文摘要：

目的探讨蛋白激酶PI3K／Akt信号途径在肝组织再生中的作用．方法采用切除小鼠肝组织2／3的部分切除法，制成小鼠肝再生模型；小鼠体内肝组织P13K的活性用腹腔注射Wortmannin来抑制．分别采用western blot和RT-RCR方法检测Akt磷酸化水平和增殖细胞核抗原PCNA mRNA表达．结果小鼠肝组织部分切除48h后，PCNA基因表达显著升高，表明肝组织再生发生．小鼠肝组织部分切除24h后，Akt磷酸化水平明显升高．PI3K特异性抑制剂wortmannin显著抑制Akt磷酸化水平和PCNA基因表达．结论PI3K／Akt信号途径在小鼠肝组织再生过程中起重要作用．

英文摘要：

Objective To study the role of PI3K/Akt pathway in liver regeneration in mice. Methods The mouse model of liver regeneration was made by two-third partial hepatectomy （PH）. To inhibit PI3K activity in mice, wortmannin was given intraperitoneally. The phosphorylation of Akt was measured by Western blotting; The mRNA levels of proliferation cell nublear antigen PCNA were detected by RT-PCR. Results The expression of PCNA markedly increased after 48 h of PH, indicating that the liver regeneration oecured. The pho- sphorylation of Akt increased after 24 h of PH. Wortmannin, a specific inhibitor of PI3K significantly inhibited the phosphorylation of Akt as well as the expression of PCNA. Conclusion PI3K/Akt pathway plays an important role in liver regeneration in mice.